Aerosol exposure biotesting for package integrity testing

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dc.contributor.authorKeller, Scott W.en
dc.contributor.committeememberMarcy, Joseph E.en
dc.contributor.committeememberHackney, Cameron Rajen
dc.contributor.committeememberBlakistone, Barbara A.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:40:04Zen
dc.date.adate2009-07-10en
dc.date.available2014-03-14T21:40:04Zen
dc.date.issued1995-01-05en
dc.date.rdate2009-07-10en
dc.date.sdate2009-07-10en
dc.description.abstractThe objective of this study was to determine how hole diameter, channel length, test organism motility, concentration and aerosol exposure time affected microbiological contamination of sealed flexible pouches. Nickel microtubes with 10 μm and 20μm hole diameters and lengths of 5 mm and 10 mm were used in various combinations to create seal defects in 128 retortable pouches. A 119,911 cm³, exposure chamber was used to distribute an aerosol with a particle size of 2.68 μm, infected with motile and isogenically mutated nonmotile <i>Pseudomonas fragi</i> TM 849 in concentrations of 10² or 10<sup>6</sup> cells/mL. Fifteen and 30 minute aerosol exposure times were used. Six pouches tested positive for test organism growth after a 72 hour incubation period. Pouch contamination via microbial ingress was significant (P < .05) for test organism motility (motile) and concentration (10<sup>6</sup> cells/mL).en
dc.description.degreeMaster of Scienceen
dc.format.extentx, 88 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07102009-040603en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07102009-040603/en
dc.identifier.urihttp://hdl.handle.net/10919/43643en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1995.K455.pdfen
dc.relation.isformatofOCLC# 34185585en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcontaminationen
dc.subject.lccLD5655.V855 1995.K455en
dc.titleAerosol exposure biotesting for package integrity testingen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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