The effects of Nd:YAG laser cyclophotocoagulation on corneal sensitivity, intraocular pressure, aqueous tear production and corneal nerve morphology in the canine eye

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dc.contributor.authorWeigt, Anne Kelleyen
dc.contributor.committeechairHerring, Ian P.en
dc.contributor.committeememberPickett, J. Phillipen
dc.contributor.committeememberMarfurt, Carl F.en
dc.contributor.committeememberDuncan, Robert B. Jr.en
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:40:02Zen
dc.date.adate2001-06-26en
dc.date.available2014-03-14T20:40:02Zen
dc.date.issued2001-06-04en
dc.date.rdate2002-06-26en
dc.date.sdate2001-06-15en
dc.description.abstractCorneal ulceration with prolonged healing following Nd:YAG laser cyclophotocoagulation in dogs is a frequent complication. It is hypothesized that these corneal ulcerations may be a form of neurotrophic keratitis due to laser-induced damage to corneal innervation. Fifteen clinically normal dogs had the neodymium:yttrium aluminum garnet(Nd:YAG) laser cyclophotocoagulation performed on the left eye. Each treated eye received 100 Joules of laser energy. Corneal touch threshold (CTT) and Schirmer I tear tests (STT) were performed before the surgery and on days 1,3,5,7,9,11, and 13 post-laser treatment. Applanation tonometry was performed before surgery and twice daily for 14 days post-laser treatment. Eyes were enucleated after 14 days and corneal nerves were stained using a gold chloride technique. Major nerve bundles entering the cornea were quantitated by quadrant, using camera lucida reproductions. Nerve bundle diameters were measured using NIH image computer software on computer-scanned images. Statistical methods included repeated values for analysis of variance for CTT, STT and IOP, and a paired t-test for nerve diameters and bundles. All laser treated eyes had significantly higher CTTs (P<0.05) compared to control eyes for all measurements. Six out of fifteen dogs had evidence of ulcerative keratitis. Intraocular pressure was significantly lower in laser treated eyes compared to control eyes in the a.m. on days 2-9,and 14, and in the p.m. on days 2-11 using a Bonferroni-corrected alpha level (P<0.0039). A significant decrease of one nerve bundle per corneal quadrant was found between the laser treated and control eyes. There was no significant difference in STT or nerve bundle diameters between laser treated and control eyes. Nd:YAG laser cyclophotocoagulation effectively reduces IOP while increasing CTT. The procedure also causes a significant decrease in the number of major nerve bundles entering the cornea, but has no effect on the diameter of those bundles. These findings support the hypothesis that nerve damage and corneal hypoesthesia are etiologic factors in ulcerative keratitis following Nd:YAG laser cyclophotocoagulation.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-06152001-152749en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06152001-152749/en
dc.identifier.urihttp://hdl.handle.net/10919/33583en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartmasters.-ed.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcorneal sensitivityen
dc.subjectaqueous tear productionen
dc.subjectintraocular pressureen
dc.subjectcorneal touch thresholden
dc.subjectcorneal nerve morphologyen
dc.subjectdogen
dc.subjectcyclophotocoagulationen
dc.titleThe effects of Nd:YAG laser cyclophotocoagulation on corneal sensitivity, intraocular pressure, aqueous tear production and corneal nerve morphology in the canine eyeen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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