Probiotic Lactobacillus rhamnosus GG mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestine

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dc.contributor.authorWu, Shaopingen
dc.contributor.authorYuan, Lijuanen
dc.contributor.authorZhang, Yongguoen
dc.contributor.authorLiu, Fangningen
dc.contributor.authorLi, Guohuaen
dc.contributor.authorWen, Keen
dc.contributor.authorKocher, Jacoben
dc.contributor.authorYang, Xingdongen
dc.contributor.authorSun, Junen
dc.date.accessioned2013-08-09T23:09:38Zen
dc.date.available2013-08-09T23:09:38Zen
dc.date.issued2013-08-07en
dc.date.updated2013-08-09T23:09:38Zen
dc.description.abstractBackground Human rotavirus (HRV) is the most important cause of severe diarrhea in infants and young children. Probiotic Lactobacillus rhamnosus GG (LGG) reduces rotavirus infection and diarrhea. However, the molecular mechanisms of LGG-mediated protection from rotavirus infection are poorly understood. Autophagy plays an essential role in responses to microbial pathogens. However, the role of autophagy in HRV infection and LGG treatment is unknown. We hypothesize that rotavirus gastroenteritis activates autophagy and that LGG suppresses virus-induced autophagy and prevents intestinal damage in infected piglets. Methods We used LGG feeding to combat viral gastroenteritis in the gnotobiotic pig model of virulent HRV infection. Results We found that LGG feeding did not increase autophagy, whereas virus infection induced autophagy in the piglet intestine. Virus infection increased the protein levels of the autophagy markers ATG16L1 and Beclin-1 and the autophagy regulator mTOR. LGG treatment during viral gastroenteritis reduced autophagy marker expression to normal levels, induced apoptosis and partially prevented virus-induced tissue damage. Conclusion Our study provides new insights into virus-induced autophagy and LGG suppression of uncontrolled autophagy and intestinal injury. A better understanding of the antiviral activity of LGG will lead to novel therapeutic strategies for infant infectious diseases.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGut Pathogens. 2013 Aug 07;5(1):22en
dc.identifier.doihttps://doi.org/10.1186/1757-4749-5-22en
dc.identifier.urihttp://hdl.handle.net/10919/23693en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderShaoping Wu et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleProbiotic Lactobacillus rhamnosus GG mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestineen
dc.title.serialGut Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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