Inactivation of Venezuelan Equine Encephalitis Virus Genome Using Two Methods

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dc.contributor.authorBehnia, Mahgolen
dc.contributor.authorBaer, Alanen
dc.contributor.authorSkidmore, Andrew M.en
dc.contributor.authorLehman, Caitlin W.en
dc.contributor.authorBracci, Nicole R.en
dc.contributor.authorKehn-Hall, Kyleneen
dc.contributor.authorBradfute, Steven B.en
dc.date.accessioned2022-02-11T16:06:13Zen
dc.date.available2022-02-11T16:06:13Zen
dc.date.issued2022-01-28en
dc.date.updated2022-02-11T14:46:23Zen
dc.description.abstractVenezuelan equine encephalitis virus (VEEV) is an Alphavirus in the Togaviridae family of positive-strand RNA viruses. The viral genome of positive-strand RNA viruses is infectious, as it produces infectious virus upon introduction into a cell. VEEV is a select agent and samples containing viral RNA are subject to additional regulations due to their infectious nature. Therefore, RNA isolated from cells infected with BSL-3 select agent strains of VEEV or other positive-strand viruses must be inactivated before removal from high-containment laboratories. In this study, we tested the inactivation of the viral genome after RNA fragmentation or cDNA synthesis, using the Trinidad Donkey and TC-83 strains of VEEV. We successfully inactivated VEEV genomic RNA utilizing these two protocols. Our cDNA synthesis method also inactivated the genomic RNA of eastern and western equine encephalitis viruses (EEEV and WEEV). We also tested whether the purified VEEV genomic RNA can produce infectious virions in the absence of transfection. Our result showed the inability of the viral genome to cause infection without being transfected into the cells. Overall, this work introduces RNA fragmentation and cDNA synthesis as reliable methods for the inactivation of samples containing the genomes of positive-strand RNA viruses.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBehnia, M.; Baer, A.; Skidmore, A.M.; Lehman, C.W.; Bracci, N.; Kehn-Hall, K.; Bradfute, S.B. Inactivation of Venezuelan Equine Encephalitis Virus Genome Using Two Methods. Viruses 2022, 14, 272.en
dc.identifier.doihttps://doi.org/10.3390/v14020272en
dc.identifier.urihttp://hdl.handle.net/10919/108288en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectencephalitisen
dc.subjectVenezuelan equine encephalitis virusen
dc.subjectRNA fragmentationen
dc.subjectcDNA synthesisen
dc.subjectviral genome inactivationen
dc.titleInactivation of Venezuelan Equine Encephalitis Virus Genome Using Two Methodsen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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