Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types

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dc.contributor.authorBarclay, R. A.en
dc.contributor.authorAkhrymuk, Ivan V.en
dc.contributor.authorPatnaik, A.en
dc.contributor.authorCallahan, Victoriaen
dc.contributor.authorLehman, C.en
dc.contributor.authorAndersen, P.en
dc.contributor.authorBarbero, R.en
dc.contributor.authorBarksdale, S.en
dc.contributor.authorDunlap, R.en
dc.contributor.authorGoldfarb, D.en
dc.contributor.authorJones-Roe, T.en
dc.contributor.authorKelly, R.en
dc.contributor.authorKim, B.en
dc.contributor.authorMiao, S.en
dc.contributor.authorMunns, A.en
dc.contributor.authorMunns, D.en
dc.contributor.authorPatel, S.en
dc.contributor.authorPorter, E.en
dc.contributor.authorRamsey, R.en
dc.contributor.authorSahoo, S.en
dc.contributor.authorSwahn, O.en
dc.contributor.authorWarsh, J.en
dc.contributor.authorKehn-Hall, Kyleneen
dc.contributor.authorLepene, Benjaminen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-08-26T12:41:12Zen
dc.date.available2021-08-26T12:41:12Zen
dc.date.issued2020-12-01en
dc.date.updated2021-08-26T12:41:07Zen
dc.description.abstractHere we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from contrived transport medium and saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus from 1 mL samples prior to RNA extraction, substantially improving detection of virus using real-time RT-PCR across a range of viral titers (100–1,000,000 viral copies/mL) and enabling detection of virus using the 2019 nCoV CDC EUA Kit down to 100 viral copies/mL. This method is compatible with commercially available nucleic acid extraction kits (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off the particle, allowing a sample processing time of 10 min. We furthermore tested our method in transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2, showing that our method not only correctly identified all positive samples but also substantially improved detection of the virus in low viral load samples. The average improvement in cycle threshold value across all viral titers tested was 3.1. Finally, we illustrate that our method could potentially be used to enable pooled testing, as we observed considerable improvement in the detection of SARS-CoV-2 RNA from sample volumes of up to 10 mL.en
dc.description.versionPublished versionen
dc.format.extentPages 22425en
dc.format.mimetypeapplication/pdfen
dc.identifier22425 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s41598-020-78771-8en
dc.identifier.eissn2045-2322en
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.orcidKehn-Hall, Kylene [0000-0001-8036-7213]en
dc.identifier.other10.1038/s41598-020-78771-8 (PII)en
dc.identifier.pmid33380736en
dc.identifier.urihttp://hdl.handle.net/10919/104715en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/33380736en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject.meshNasopharynxen
dc.subject.meshSalivaen
dc.subject.meshHumansen
dc.subject.meshRNA, Viralen
dc.subject.meshHydrogelsen
dc.subject.meshDiagnostic Tests, Routineen
dc.subject.meshSpecimen Handlingen
dc.subject.meshViral Loaden
dc.subject.meshSensitivity and Specificityen
dc.subject.meshReal-Time Polymerase Chain Reactionen
dc.subject.meshCOVID-19en
dc.subject.meshSARS-CoV-2en
dc.subject.meshCOVID-19 Testingen
dc.titleHydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample typesen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2020-11-19en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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