Endotoxin-induced microRNA expression in equine peripheral blood mononuclear cells

Simple item page
dc.contributor.authorParkinson, Nicholas J.en
dc.contributor.committeechairBuechner-Maxwell, Virginia A.en
dc.contributor.committeememberPleasant, R. Scotten
dc.contributor.committeememberWitonsky, Sharon G.en
dc.contributor.committeememberAhmed, S. Ansaren
dc.contributor.departmentBiomedical and Veterinary Sciencesen
dc.date.accessioned2018-01-14T07:00:41Zen
dc.date.available2018-01-14T07:00:41Zen
dc.date.issued2016-07-22en
dc.description.abstractThe innate immune response to lipopolysaccharide (LPS) mediated by toll-like receptor 4 (TLR4) contributes substantially to the morbidity of equine gastrointestinal disease, neonatal sepsis and other diseases. MicroRNAs (miRNAs), small non-coding RNA molecules acting as post-transcriptional regulators of gene expression, have key roles in TLR4 signaling regulation in other species. The central hypothesis of this study was that LPS induces differential expression of miRNAs in equine peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from healthy adult horses and cultured with LPS or medium only for 2, 4 and 8 hours. Concentrations of inflammatory cytokines were measured in supernatants by immunoassay. Illumina Next-Generation Sequencing of the miRNA transcriptome was performed in PBMCs at 0, 2 and 4 hours. Selected expression changes were verified by qRT-PCR. 327 mature miRNAs were detected in equine PBMCs. Only miR-155 was significantly upregulated by LPS. 9 miRNAs showed statistically significant expression changes with time. Tumor necrosis factor-α concentration was significantly higher in supernatants from LPS-treated cells than controls from 2 hours, while interleukin-10 and interferon-γ were increased at 8 hours. miR-155 expression was correlated to all three cytokines. These data provide a foundation for future research into miRNA involvement in equine inflammatory responses. miR-155 is the principal LPS-induced miRNA in horses. Bioinformatic target predictions support roles in regulation of innate and adaptive immune responses including TLR4 signaling, as in humans. It is thus likely to influence the acute inflammatory response to LPS. Further research will be necessary to establish its role in naturally occurring disease.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:8560en
dc.identifier.urihttp://hdl.handle.net/10919/81766en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectendotoxemiaen
dc.subjectHorsesen
dc.subjectinnate immunityen
dc.subjectlipopolysaccharideen
dc.subjectmicroRNAen
dc.subjectNext Generation Sequencingen
dc.subjecttoll-like receptoren
dc.titleEndotoxin-induced microRNA expression in equine peripheral blood mononuclear cellsen
dc.typeThesisen
thesis.degree.disciplineBiomedical and Veterinary Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 3 of 3
Name:
Parkinson_NJ_T_2016_support_2.xlsx
Size:
101.07 KB
Format:
Microsoft Excel XML
Description:
Supporting documents
Download
Thumbnail Image
Name:
Parkinson_NJ_T_2016.pdf
Size:
2.96 MB
Format:
Adobe Portable Document Format
Download
Name:
Parkinson_NJ_T_2016_support_3.xlsx
Size:
26.44 KB
Format:
Microsoft Excel XML
Description:
Supporting documents
Download

Collections

Masters Theses