Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation

The transcription factor Krüppel homolog 1 (Kr-h1) is crucial in multiple reproductive processes of Aedes aegypti mosquitoes, including previtellogenesis, vitellogenesis, and oogenesis. This study explores the interaction between Kr-h1 and its potential associated proteins (KAPs), with a specific focus on the dimerization partner (DP-1), and how this interaction regulates gene expression pathways critical for mosquito reproduction. Utilizing RNA interference (RNAi), the research identifies DP-1 as a significant regulator of follicle growth post-eclosion (PE), highlighting its vital role in the mosquito reproductive regulatory pathway. The experimental approach included RNAi-mediated knockdown of DP-1, accompanied by evaluations using quantitative PCR (qPCR), Western blotting (WB), co-immunoprecipitation (Co-IP), follicle length measurement, and egg counting to assess the role of DP-1 in reproductive functions. For the first time, the inhibition of DP-1 expression was found to significantly impede A. aegypti follicular development. The elucidation of the mechanistic roles of Kr-h1 and DP-1 provides valuable insights that could lead to innovative strategies for mosquito population control and effective disease vector management.