The evolution of hydrogen sulfide by Gluconobacter species

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dc.contributor.authorSwartwood, Suzanne Christineen
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2014-03-14T21:29:02Zen
dc.date.adate2009-02-13en
dc.date.available2014-03-14T21:29:02Zen
dc.date.issued1995en
dc.date.rdate2009-02-13en
dc.date.sdate2009-02-13en
dc.description.abstractPrevious studies demonstrate that members of the strictly aerobic genus Gluconobacter produce detectable quantities of hydrogen sulfide (H₂S) when incubated in SYP medium (5% sorbitol, 1% yeast extract, and 1% peptone) containing thiosulfate. This finding is puzzling, since the microbial evolution of H₂S is characteristic of anaerobic or facultative bacteria. The goal of this research was to determine the physiological role of H₂S evolution for the gluconobacters. A methylene blue method was used to quantify the amount of H₂S evolved from cultures grown aerobically for 3 days at 28°C. Five of the six tested strains of gluconobacter evolved from 6 to 68 μg of H₂S. Strains which grew to a higher density (> 300 μg cell protein/ml ) evolved between 10 and 68 μg of H₂S. Strains which grew to a lesser extent (< 140 μg cell protein/ml ) evolved no more than 6 μg of H₂S. Uninoculated SYP medium containing 1% thiosulfate showed no evidence of H₂S evolution; however, sterile SYP medium with decreasing concentrations of yeast extract and peptone showed increasing amounts of H₂S evolved. When SYP medium was exhausted by gluconobacter growth for 72 hours, then supplemented with thiosulfate, filter sterilized, and incubated for 3 days at 28°C, these sterile solutions evolved over 400 μg of H₂S. A drop in pH. similar to that which occurs during gluconobacter growth, is not sufficient to evolve H₂S. My results to date suggest that H₂S evolution results not from gluconobacter metabolism, but rather from spontaneous decomposition of thiosulfate and the depletion of media components during growth.en
dc.description.degreeMaster of Scienceen
dc.format.extentix, 53 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-02132009-171359en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-02132009-171359/en
dc.identifier.urihttp://hdl.handle.net/10919/41046en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1995.S937.pdfen
dc.relation.isformatofOCLC# 34103571en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1995.S937en
dc.titleThe evolution of hydrogen sulfide by Gluconobacter speciesen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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