Pseudomonas spp. Isolated from the Soybean Nodule Interior Promote Soybean Growth upon Field Amendment

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dc.contributor.authorDoyle, Connor Patricken
dc.contributor.committeechairWilliams, Mark A.en
dc.contributor.committeememberVinatzer, Boris A.en
dc.contributor.committeememberFike, John H.en
dc.contributor.departmentCrop and Soil Environmental Sciencesen
dc.date.accessioned2022-09-01T08:00:30Zen
dc.date.available2022-09-01T08:00:30Zen
dc.date.issued2022-08-31en
dc.description.abstractDiazotrophic microbes reside in soybean nodules; however, other non-nitrogen fixing bacteria are a part of the interior nodule microbiome. Results from a previous greenhouse study show that a novel species of Pseudomonas associates with soybean nodules as a plant-growth promoting rhizobacteria (PGPR). This study observes the soybean growth promoting potential of Pseudomonas spp. in a field setting. Additionally, this study observed differences in soybean growth promotion based on amending the plant with isolated strains or a mixed culture of the species' strains. Two cultivars of soybean (Asgrow AG46X6 and Pioneer P48A60X) were either amended with isolated strains of the novel Pseudomonas spp. (referred to as PAMW1 and BUMW2 in this study), a mix of the two strains, or an uninoculated control. The study recorded measurements to observe growth, yield, and nitrogen fixation differences. The study uses two-way factorial ANOVAs and non-parametric, multivariate analyses to determine differences in growth promotion among samples. Soybean amended with PAMW1 has greater shoot mass, biomass, and height than other treatments. Through nonmetric multidimensional scaling (NMS), samples amended with a mixed culture or PAMW1 may be different regarding growth promotion relative to the non-amended samples. Univariate results support the hypothesis that the novel Pseudomonas spp. benefit soybean in a field setting. However, it is inconclusive whether a mixed culture amendment of multiple strains alters the overall growth promotion of soybean compared to samples amended with isolated strains.en
dc.description.abstractgeneralSoil hosts a relatively abundant and diverse community of microorganisms. Moreover, the area of soil that interacts closely with plant roots and their associated exudates, called the rhizosphere, has a significantly greater microbial abundance than surrounding bulk soil. Interactions between microbes and the plant often promote plant growth because of secondary metabolites produced by these beneficial microbes. One particular bacterial species, belonging to the Pseudomonas genus, was discovered and extracted from the soybean nodule interior. Nitrogen-fixing bacteria predominantly reside in the soybean nodule, yet this microorganism cannot fix nitrogen. Although trace amounts of non-nitrogen-fixing bacteria reside in the soybean nodule, this novel species has a relatively high abundance. This study determines the benefits of this species in the soybean nodule. Following positive results in a greenhouse study, this field experiment observes variance in soybean growth and productivity based on their received bacterial amendment. For this study, two soybean cultivars were either amended with an isolated strain of this species, a mix of the two strains, or left uninoculated to serve as a control. Numerous recorded measurements serve as indices of soybean growth and productivity. The results suggest that this novel Pseudomonas species benefits the plant by significantly improving biomass. With further research, this species can potentially serve as an environmentally sensitive and sustainable alternative to fertilizers through its ability to promote soybean growth. en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:35341en
dc.identifier.urihttp://hdl.handle.net/10919/111684en
dc.language.isoenen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPseudomonas spp.en
dc.subjectPlant Growth-Promoting Rhizobacteria (PGPR)en
dc.subjectSoybeanen
dc.subjectGrowthen
dc.subjectBradyrhizobium spp.en
dc.subjectNodule Sterilizationen
dc.subjectBacterial Extractionen
dc.subjectNitrogen Fixationen
dc.subjectPhosphorus Solubilizationen
dc.subjectIndole-3-Acetic Acid (IAA)en
dc.subjectSiderophoreen
dc.subjectAnalysis of Varien
dc.titlePseudomonas spp. Isolated from the Soybean Nodule Interior Promote Soybean Growth upon Field Amendmenten
dc.typeThesisen
thesis.degree.disciplineCrop and Soil Environmental Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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