Human Rotavirus Replicates in Salivary Glands and Primes Immune Responses in Facial and Intestinal Lymphoid Tissues of Gnotobiotic Pigs

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dc.contributor.authorNyblade, Charlotteen
dc.contributor.authorZhou, Pengen
dc.contributor.authorFrazier, Maggieen
dc.contributor.authorFrazier, Annieen
dc.contributor.authorHensley, Caseyen
dc.contributor.authorFantasia-Davis, Arianaen
dc.contributor.authorShahrudin, Shabihahen
dc.contributor.authorHoffer, Mirandaen
dc.contributor.authorAgbemabiese, Chantal Amaen
dc.contributor.authorLaRue, Laurenen
dc.contributor.authorBarro, Marioen
dc.contributor.authorPatton, John T.en
dc.contributor.authorParreƱo, Vivianaen
dc.contributor.authorYuan, Lijuanen
dc.date.accessioned2023-09-27T14:46:42Zen
dc.date.available2023-09-27T14:46:42Zen
dc.date.issued2023-08-31en
dc.date.updated2023-09-27T12:36:15Zen
dc.description.abstractHuman rotavirus (HRV) is a leading cause of viral gastroenteritis in children across the globe. The virus has long been established as a pathogen of the gastrointestinal tract, targeting small intestine epithelial cells and leading to diarrhea, nausea, and vomiting. Recently, this classical infection pathway was challenged by the findings that murine strains of rotavirus can infect the salivary glands of pups and dams and transmit via saliva from pups to dams during suckling. Here, we aimed to determine if HRV was also capable of infecting salivary glands and spreading in saliva using a gnotobiotic (Gn) pig model of HRV infection and disease. Gn pigs were orally inoculated with various strains of HRV, and virus shedding was monitored for several days post-inoculation. HRV was shed nasally and in feces in all inoculated pigs. Infectious HRV was detected in the saliva of four piglets. Structural and non-structural HRV proteins, as well as the HRV genome, were detected in the intestinal and facial tissues of inoculated pigs. The pigs developed high IgM antibody responses in serum and small intestinal contents at 10 days post-inoculation. Additionally, inoculated pigs had HRV-specific IgM antibody-secreting cells present in the ileum, tonsils, and facial lymphoid tissues. Taken together, these findings indicate that HRV can replicate in salivary tissues and prime immune responses in both intestinal and facial lymphoid tissues of Gn pigs.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationNyblade, C.; Zhou, P.; Frazier, M.; Frazier, A.; Hensley, C.; Fantasia-Davis, A.; Shahrudin, S.; Hoffer, M.; Agbemabiese, C.A.; LaRue, L.; Barro, M.; Patton, J.T.; ParreƱo, V.; Yuan, L. Human Rotavirus Replicates in Salivary Glands and Primes Immune Responses in Facial and Intestinal Lymphoid Tissues of Gnotobiotic Pigs. Viruses 2023, 15, 1864.en
dc.identifier.doihttps://doi.org/10.3390/v15091864en
dc.identifier.urihttp://hdl.handle.net/10919/116359en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectrotavirusen
dc.subjectgnotobiotic pigsen
dc.subjectsalivary glandsen
dc.titleHuman Rotavirus Replicates in Salivary Glands and Primes Immune Responses in Facial and Intestinal Lymphoid Tissues of Gnotobiotic Pigsen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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