Gene Expression of Nutrient Transporters and Digestive Enzymes in the Yolk Sac Membrane and Small Intestine of the Developing Embryonic Chick

dc.contributor.authorSpeier, Jacqueline S.en
dc.contributor.committeechairWong, Eric A.en
dc.contributor.committeememberDalloul, Rami A.en
dc.contributor.committeememberMcElroy, Audrey P.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2014-03-14T21:42:53Zen
dc.date.adate2011-09-20en
dc.date.available2014-03-14T21:42:53Zen
dc.date.issued2011-07-26en
dc.date.rdate2011-09-20en
dc.date.sdate2011-08-16en
dc.description.abstractChick embryos derive nutrients from the yolk during incubation and transition to intestinal absorption posthatch. Nutrient uptake is mediated by digestive enzymes and membrane bound transporter proteins. The objective of this study was to determine expression profiles of nutrient transporters and digestive enzymes during incubation in the yolk sac membrane (YSM) and small intestine of Leghorn and Cobb chickens derived from 22–30 wk (young) and 45–50 wk (old) breeder flocks. Genes examined included peptide transporter PepT1, amino acid transporters EAAT3, CAT1, and B0AT, monosaccharide transporters SGLT1 and GLUT5, and digestive enzymes APN and SI. Expression of these genes was measured in YSM at embryonic day (e) 11, 13, 15, 17, 19, 20, and 21 and small intestine at e15, e17, e19, e20, and e21. Absolute quantification real-time PCR assessed gene expression. PepT1, APN, and B0AT expression in YSM peaked between e15 and e17 and then decreased until e21, while expression increased over time in the small intestine. SGLT1 and EAAT3 expression increased over time in the small intestine and YSM. There was minimal gene expression of SI in the YSM, while the small intestine had high expression. GLUT5 and CAT1 expression decreased in the YSM, while peaking at e19 then decreasing in the small intestine. Breed and flock age affected expression levels in some genes. These results demonstrate that these genes show tissue- and development-specific patterns of expression and that the YSM expresses many digestive enzymes and nutrient transporters associated with the small intestine.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-08162011-124927en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08162011-124927/en
dc.identifier.urihttp://hdl.handle.net/10919/44254en
dc.publisherVirginia Techen
dc.relation.haspartSpeier_JS_T_2011.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectDigestive Enzymesen
dc.subjectNutrient Transportersen
dc.subjectIntestineen
dc.subjectYolk Sac Membraneen
dc.titleGene Expression of Nutrient Transporters and Digestive Enzymes in the Yolk Sac Membrane and Small Intestine of the Developing Embryonic Chicken
dc.typeThesisen
thesis.degree.disciplineAnimal and Poultry Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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