Effects of La Crosse virus infection on the host-seeking behavior and levels of two neurotransmitters in Aedes triseriatus
| dc.contributor.author | Yang, Fan | en |
| dc.contributor.author | Chan, Kevin K. | en |
| dc.contributor.author | Brewster, Carlyle C. | en |
| dc.contributor.author | Paulson, Sally L. | en |
| dc.contributor.department | Entomology | en |
| dc.date.accessioned | 2019-08-12T14:13:48Z | en |
| dc.date.available | 2019-08-12T14:13:48Z | en |
| dc.date.issued | 2019-08-09 | en |
| dc.date.updated | 2019-08-11T03:21:13Z | en |
| dc.description.abstract | Background La Crosse virus (LACV) infection has been shown to manipulate the blood-feeding behaviors of its main vector, Aedes triseriatus. Here, we investigated the effects of virus infection on serotonin and dopamine and their potential roles in host-seeking. In mosquitoes, serotonin depletion has been shown to interfere with blood-feeding but not host-seeking. Dopamine depletion does not affect either blood-feeding or host-seeking; elevations of dopamine, however, has been shown to inhibit host-seeking. The purpose of this study was to determine the effects of LACV infection on the host-seeking behavior of and neurotransmitter levels in Ae. triseriatus. Methods Host-seeking behavior was evaluated using a uni-port olfactometer and a membrane feeder assay. Levels of serotonin and dopamine in infected and control mosquito heads were measured using HPLC-ED. Results Infection with LACV significantly inhibited the activation and attraction of Ae. triseriatus females to a host. A higher proportion of uninfected Ae. triseriatus females were activated by the presence of a host compared to infected mosquitoes and more uninfected mosquitoes were full responders (95.7%) compared to infected ones (91.1%). However, infection with LACV did not significantly affect the landing, probing, or blood-feeding rates of female mosquitoes. LACV-infected mosquitoes had lower serotonin levels than controls (104.5 vs 138.3 pg/head) while the dopamine levels were not affected by infection status (282.3 vs 237 pg/head). Conclusions Our work suggests that virus-induced reduction of serotonin is related to previously reported blood-feeding alterations in LACV-infected mosquitoes and could lead to enhanced transmission and increased vectorial capacity. In addition, some aspects of host-seeking were inhibited by virus infection. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Parasites & Vectors. 2019 Aug 09;12(1):397 | en |
| dc.identifier.doi | https://doi.org/10.1186/s13071-019-3658-6 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/93027 | en |
| dc.language.iso | en | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.holder | The Author(s) | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.title | Effects of La Crosse virus infection on the host-seeking behavior and levels of two neurotransmitters in Aedes triseriatus | en |
| dc.title.serial | Parasites & Vectors | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |