A Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)

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dc.contributor.authorKoechlein, David Jacoben
dc.contributor.committeechairKrieg, Noel R.en
dc.contributor.committeememberYousten, Allan A.en
dc.contributor.committeememberClaus, George Williamen
dc.contributor.departmentBiologyen
dc.date.accessioned2014-03-14T20:47:35Zen
dc.date.adate1998-11-11en
dc.date.available2014-03-14T20:47:35Zen
dc.date.issued1998-09-17en
dc.date.rdate1998-11-11en
dc.date.sdate1998-12-07en
dc.description.abstractFollowing active growth, the aquatic gram-negative rod Prolinoborus fasciculus (Aquaspirillum fasciculus) exhibited a mass conversion from its culturable rod form to a nonculturable coccoid form. Chloramphenicol did not prevent the conversion. Attempts to obtain variants that would not convert to the coccoid form were unsuccessful. Although the coccoid form fluoresced with acridine orange, agarose gel electrophoresis revealed extensive ribosomal RNA degradation. Poly-Ã -hydroxybutyrate, abundant in the vegetative rods, was not detectable in the coccoid cells. The results suggest that the coccoid form of A. fasciculus is a degenerative form rather than part of a life cycle.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-110798-012740en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-110798-012740/en
dc.identifier.urihttp://hdl.handle.net/10919/35624en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartvitae.PDFen
dc.relation.haspartthesis.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectRNAen
dc.subjectacridine orangeen
dc.subjectViable but nonculturableen
dc.subjectcoccoid bodiesen
dc.subjectVBNCen
dc.titleA Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)en
dc.typeThesisen
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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