Flavor and Quality Characteristics of Two Primocane-bearing Blackberry Cultivars as Impacted by Foliar and Shade Application

Blackberry (Rubus spp.) is a popular fruit due to its delightful taste and notable health benefits. With a growing demand for regional blackberry production, this study aims to provide Virginia blackberry growers with information on the effectiveness of pre-harvest foliar treatments and shade application on yield, white drupelet disorder, post-harvest attributes, and the aroma profile of two blackberry cultivars, Prime-Ark® Traveler and Prime-Ark® Freedom. The field study was repeated in the 2021 and 2022 growing seasons at the Hampton Roads Agricultural Research and Extension Center in Virginia Beach, VA, in a completely randomized design. Grower standard control (GSC), shade cloth with 30% light reduction (SHA), calcium (CAL), and salicylic acid (SAL) foliar applications were randomly assigned to each variety. Fruit yield and physicochemical attributes of the blackberry were collected and analyzed, and aroma-active compounds in blackberries were identified by use of headspace-solid-phase microextraction-gas chromatography-mass spectrometry-olfaction (HS-SPME-GC-MS-O). Shade cloth significantly reduced the white drupelet disorder (WDD) in Prime-Ark® Freedom but it also reduced the total soluble solid content (°Bx) and °Bx/% titratable acidity (a ratio indicates the sweet and sour balance for fruit) for both cultivars. No significant improvement was found in the TSS, TA, firmness and yield of blackberries treated with CAL and SAL. In total, 16 consistent aroma-active compounds were found across treatments for both varieties and growing seasons. Foliar and shade application did not alter the aroma profile of either blackberry cultivar. However, higher volatile contents were found in 2021 than in 2022, possibly due to climate variation. A clear distinction on aroma profiles of the above two cultivars were also observed: PrimeArk® Freedom was higher in compounds possessing "fruity" and "floral" notes, while PrimeArk® Traveler featured more "green" and "fresh" characteristics. Regional berry growers should be more conservative when adopting foliar and shade applications due to potential seasonal variations surpassing the significance of agronomic treatments.