Tissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsis

Simple item page
dc.contributor.authorKositsup, Boonthidaen
dc.contributor.committeechairBeers, Eric P.en
dc.contributor.committeememberWelbaum, Gregory E.en
dc.contributor.committeememberHess, John L.en
dc.contributor.departmentHorticultureen
dc.date.accessioned2014-03-14T20:34:18Zen
dc.date.adate2000-04-28en
dc.date.available2014-03-14T20:34:18Zen
dc.date.issued2000-04-18en
dc.date.rdate2001-04-28en
dc.date.sdate2000-04-26en
dc.description.abstractCysteine proteases are associated with xylem tracheary element differentiation. XCP1 was recently identified as a xylem-specific cysteine protease in Arabidopsis (Zhao, et al., 2000). For this study a recombinant polyhistidine-tagged XCP1 (XCP1H6) was expressed and purified from an E. coli expression system. A polyclonal anti-XCP1 antibody was produced using purified XCP1H6. Immunoblot analysis of a developmental time course of xylem and bark protein extracted from root-hypocotyl segments demonstrated that XCP1 was expressed in xylem only. Further analysis under optimized immunoblot conditions, however, revealed that anti-XCP1 antibody reacted with protein present in both xylem and bark. The vast majority of immunoreactivity, however, was restricted to xylem. Cell-type localization of GUS expression under the control of a putative XCP1 promoter indicated that the XCP1 promoter specifies expression of XCP1 in tracheary elements in leaves, stems, roots and flowers. XCP1 promoter-driven GUS activity was not associated with senescing tissues.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-04262000-17260046en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04262000-17260046/en
dc.identifier.urihttp://hdl.handle.net/10919/31923en
dc.publisherVirginia Techen
dc.relation.haspartthesisbk.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectprogrammed cell deathen
dc.subjectcysteine proteaseen
dc.subjectArabidopsis thalianaen
dc.subjecttracheary elementen
dc.subjectxylemen
dc.subjectlocalizationen
dc.titleTissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsisen
dc.typeThesisen
thesis.degree.disciplineHorticultureen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesisbk.pdf
Size:
921.21 KB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses