Exploring the immunogenicity of an insect-specific virus vectored Zika vaccine candidate
dc.contributor.author | Tanelus, Manette | en |
dc.contributor.author | López, Krisangel | en |
dc.contributor.author | Smith, Shaan | en |
dc.contributor.author | Muller, John A. | en |
dc.contributor.author | Porier, Danielle L. | en |
dc.contributor.author | Auguste, Dawn I. | en |
dc.contributor.author | Stone, William B. | en |
dc.contributor.author | Paulson, Sally L. | en |
dc.contributor.author | Auguste, A. Jonathan | en |
dc.date.accessioned | 2024-01-16T18:51:56Z | en |
dc.date.available | 2024-01-16T18:51:56Z | en |
dc.date.issued | 2023-12-01 | en |
dc.description.abstract | Zika virus (ZIKV) is an important re-emerging flavivirus that presents a significant threat to human health worldwide. Despite its importance, no vaccines are approved for use in humans. Insect-specific flaviviruses (ISFVs) have recently garnered attention as an antigen presentation platform for vaccine development and diagnostic applications. Here, we further explore the safety, immunogenicity, and efficacy of a chimeric ISFV-Zika vaccine candidate, designated Aripo-Zika (ARPV/ZIKV). Our results show a near-linear relationship between increased dose and immunogenicity, with 1011 genome copies (i.e., 108 focus forming units) being the minimum dose required for protection from ZIKV-induced morbidity and mortality in mice. Including boosters did not significantly increase the short-term efficacy of ARPV/ZIKV-vaccinated mice. We also show that weanling mice derived from ARPV/ZIKV-vaccinated dams were completely protected from ZIKV-induced morbidity and mortality upon challenge, suggesting efficient transfer of maternally-derived protective antibodies. Finally, in vitro coinfection studies of ZIKV with Aripo virus (ARPV) and ARPV/ZIKV in African green monkey kidney cells (i.e., Vero-76) showed that ARPV and ARPV/ZIKV remain incapable of replication in vertebrate cells, despite the presence of active ZIKV replication. Altogether, our data continue to support ISFV-based vaccines, and specifically the ARPV backbone is a safe, immunogenic and effective vaccine strategy for flaviviruses. | en |
dc.description.version | Published version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier | 19948 (Article number) | en |
dc.identifier.doi | https://doi.org/10.1038/s41598-023-47086-9 | en |
dc.identifier.eissn | 2045-2322 | en |
dc.identifier.issn | 2045-2322 | en |
dc.identifier.issue | 1 | en |
dc.identifier.orcid | Auguste, Albert [0000-0001-9048-0041] | en |
dc.identifier.orcid | Muller, John [0000-0001-5754-5560] | en |
dc.identifier.orcid | Paulson, Sally [0000-0003-4206-9279] | en |
dc.identifier.pmid | 37968443 | en |
dc.identifier.uri | https://hdl.handle.net/10919/117365 | en |
dc.identifier.volume | 13 | en |
dc.language.iso | en | en |
dc.publisher | Springer | en |
dc.relation.uri | https://www.ncbi.nlm.nih.gov/pubmed/37968443 | en |
dc.rights | Creative Commons Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Infectious Diseases | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Humans | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Viral Vaccines | en |
dc.subject.mesh | Antibodies, Viral | en |
dc.subject.mesh | Antibodies, Neutralizing | en |
dc.subject.mesh | Zika Virus | en |
dc.subject.mesh | Zika Virus Infection | en |
dc.subject.mesh | Immunogenicity, Vaccine | en |
dc.subject.mesh | Chlorocebus aethiops | en |
dc.title | Exploring the immunogenicity of an insect-specific virus vectored Zika vaccine candidate | en |
dc.title.serial | Scientific Reports | en |
dc.type | Article - Refereed | en |
dc.type.dcmitype | Text | en |
dc.type.other | Journal Article | en |
dcterms.dateAccepted | 2023-11-08 | en |
pubs.organisational-group | /Virginia Tech | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Entomology | en |
pubs.organisational-group | /Virginia Tech/All T&R Faculty | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty | en |