Exploring the immunogenicity of an insect-specific virus vectored Zika vaccine candidate

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dc.contributor.authorTanelus, Manetteen
dc.contributor.authorLópez, Krisangelen
dc.contributor.authorSmith, Shaanen
dc.contributor.authorMuller, John A.en
dc.contributor.authorPorier, Danielle L.en
dc.contributor.authorAuguste, Dawn I.en
dc.contributor.authorStone, William B.en
dc.contributor.authorPaulson, Sally L.en
dc.contributor.authorAuguste, A. Jonathanen
dc.date.accessioned2024-01-16T18:51:56Zen
dc.date.available2024-01-16T18:51:56Zen
dc.date.issued2023-12-01en
dc.description.abstractZika virus (ZIKV) is an important re-emerging flavivirus that presents a significant threat to human health worldwide. Despite its importance, no vaccines are approved for use in humans. Insect-specific flaviviruses (ISFVs) have recently garnered attention as an antigen presentation platform for vaccine development and diagnostic applications. Here, we further explore the safety, immunogenicity, and efficacy of a chimeric ISFV-Zika vaccine candidate, designated Aripo-Zika (ARPV/ZIKV). Our results show a near-linear relationship between increased dose and immunogenicity, with 1011 genome copies (i.e., 108 focus forming units) being the minimum dose required for protection from ZIKV-induced morbidity and mortality in mice. Including boosters did not significantly increase the short-term efficacy of ARPV/ZIKV-vaccinated mice. We also show that weanling mice derived from ARPV/ZIKV-vaccinated dams were completely protected from ZIKV-induced morbidity and mortality upon challenge, suggesting efficient transfer of maternally-derived protective antibodies. Finally, in vitro coinfection studies of ZIKV with Aripo virus (ARPV) and ARPV/ZIKV in African green monkey kidney cells (i.e., Vero-76) showed that ARPV and ARPV/ZIKV remain incapable of replication in vertebrate cells, despite the presence of active ZIKV replication. Altogether, our data continue to support ISFV-based vaccines, and specifically the ARPV backbone is a safe, immunogenic and effective vaccine strategy for flaviviruses.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier19948 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s41598-023-47086-9en
dc.identifier.eissn2045-2322en
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.orcidAuguste, Albert [0000-0001-9048-0041]en
dc.identifier.orcidMuller, John [0000-0001-5754-5560]en
dc.identifier.orcidPaulson, Sally [0000-0003-4206-9279]en
dc.identifier.pmid37968443en
dc.identifier.urihttps://hdl.handle.net/10919/117365en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37968443en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectInfectious Diseasesen
dc.subject.meshAnimalsen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshViral Vaccinesen
dc.subject.meshAntibodies, Viralen
dc.subject.meshAntibodies, Neutralizingen
dc.subject.meshZika Virusen
dc.subject.meshZika Virus Infectionen
dc.subject.meshImmunogenicity, Vaccineen
dc.subject.meshChlorocebus aethiopsen
dc.titleExploring the immunogenicity of an insect-specific virus vectored Zika vaccine candidateen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2023-11-08en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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Scholarly Works, Center for Emerging, Zoonotic, and Arthropod-borne Pathogens (CeZAP)
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