Application of Fourier Transform Infrared Spectroscopy for Detection of Bacterial Fruit Blotch Disease

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dc.contributor.authorMargenot, Andrew J.en
dc.contributor.authorParikh, Sanjai J.en
dc.contributor.authorZhao, Bingyu Y.en
dc.contributor.authorWalcott, Ronald R.en
dc.contributor.authorWelbaum, Gregory E.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-01-16T16:41:27Zen
dc.date.available2018-01-16T16:41:27Zen
dc.date.issued2017-09-13en
dc.description.abstractBacterial fruit blotch (BFB), caused by Acidovorax citrulli affects the production of cucurbits worldwide, and causes substantial economic losses. Since cucurbit seeds are the most important source of inoculum for BFB outbreaks, seed health testing is an important component of disease management. Currently, there are no nondestructive assays that are sensitive enough to reliably detect A. citrulli-infected seeds. Fourier transform infrared spectroscopy (FTIR) may provide the sensitivity necessary to detect A. citrulli-infected seeds. Attenuated total reflectance (ATR) FTIR was evaluated for the detection of watermelon seeds infected with A. citrulli by pistil inoculation. A. citrulli cells produced a unique signature at a detection limit of approximately 105 cfu/mL. Infected, dry watermelon seeds whose embryos were infected with A. citrulli produced a different spectral profile compared to non-infected seeds. Spectral subtractions between infected and non-infected seeds suggest the potential for indirect detection of A. citrulli by altered ester C-O absorbance bands. Principal component analysis (PCA) of seeds infected with bacterial concentrations ranging from 0.001 – 0.1 OD demonstrated potential for multivariate detection of infected seeds at intermediate contamination levels (0.01 OD) relative to non-infected seeds. This separation was driven by high loading of ester C-O absorbance at frequencies ranging from 1120 - 1000 cm-1, though absorbances identified in pure A. citrulli culture were not observed. These results suggest that FTIR can be used to nondestructively detect seeds infected with moderate levels (105 cfu/mL) of A. citrulli infection.en
dc.description.notesThis was an invited 30 minute presentation at the ISSS meetingen
dc.description.notestrue (Invited?)en
dc.format.mimetypeapplication/pdfen
dc.identifier.orcidWelbaum, GE [0000-0001-8694-2856]en
dc.identifier.urihttp://hdl.handle.net/10919/81805en
dc.language.isoenen
dc.relation.ispartofThe 12th Triennial Conference of The International Society for Seed Science 10 — 14 September, 2017 • Monterey, California USAen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectBacterial fruit blotchen
dc.subjectFTIRen
dc.subjectAcidovorax citrullien
dc.subjectseed biologyen
dc.titleApplication of Fourier Transform Infrared Spectroscopy for Detection of Bacterial Fruit Blotch Diseaseen
dc.typeConference proceedingen
dc.type.dcmitypeTexten
pubs.finish-date2017-09-14en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Horticultureen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.start-date2017-09-10en

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