Molecular Prevalence, Genetic Diversity, and Tissue Tropism of Bartonella Species in Small Mammals from Yunnan Province, China
| dc.contributor.author | Han, Pei-Yu | en |
| dc.contributor.author | Xu, Fen-Hui | en |
| dc.contributor.author | Tian, Jia-Wei | en |
| dc.contributor.author | Zhao, Jun-Ying | en |
| dc.contributor.author | Yang, Ze | en |
| dc.contributor.author | Kong, Wei | en |
| dc.contributor.author | Wang, Bo | en |
| dc.contributor.author | Guo, Li-Jun | en |
| dc.contributor.author | Zhang, Yun-Zhi | en |
| dc.date.accessioned | 2024-05-10T19:16:09Z | en |
| dc.date.available | 2024-05-10T19:16:09Z | en |
| dc.date.issued | 2024-04-28 | en |
| dc.date.updated | 2024-05-10T13:17:52Z | en |
| dc.description.abstract | <i>Bartonella</i> is an intracellular parasitic zoonotic pathogen that can infect animals and cause a variety of human diseases. This study investigates <i>Bartonella</i> prevalence in small mammals in Yunnan Province, China, focusing on tissue tropism. A total of 333 small mammals were sampled from thirteen species, three orders, four families, and four genera in Heqing and Gongshan Counties. Conventional PCR and real-time quantitative PCR (qPCR) were utilized for detection and quantification, followed by bioinformatic analysis of obtained DNA sequences. Results show a 31.5% detection rate, varying across species. Notably, <i>Apodemus chevrieri, Eothenomys eleusis, Niviventer fulvescens, Rattus tanezumi, Episoriculus leucops, Anourosorex squamipes</i>, and <i>Ochotona Thibetana</i> exhibited infection rates of 44.4%, 27.7%, 100.0%, 6.3%, 60.0%, 23.5%, and 22.2%, respectively. Genetic analysis identified thirty, ten, and five strains based on <i>ssrA</i>, <i>rpoB</i>, and <i>gltA</i> genes, with nucleotide identities ranging from 92.1% to 100.0%. <i>Bartonella</i> strains were assigned to <i>B. grahamii</i>, <i>B. rochalimae</i>, <i>B. sendai</i>, <i>B. koshimizu</i>, <i>B. phoceensis</i>, <i>B. taylorii</i>, and a new species identified in <i>Episoriculus leucops</i> (GS136). Analysis of the different tissues naturally infected by <i>Bartonella</i> species revealed varied copy numbers across different tissues, with the highest load in spleen tissue. These findings underscore <i>Bartonella</i>’s diverse species and host range in Yunnan Province, highlighting the presence of extensive tissue tropism in <i>Bartonella</i> species naturally infecting small mammalian tissues. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Han, P.-Y.; Xu, F.-H.; Tian, J.-W.; Zhao, J.-Y.; Yang, Z.; Kong, W.; Wang, B.; Guo, L.-J.; Zhang, Y.-Z. Molecular Prevalence, Genetic Diversity, and Tissue Tropism of Bartonella Species in Small Mammals from Yunnan Province, China. Animals 2024, 14, 1320. | en |
| dc.identifier.doi | https://doi.org/10.3390/ani14091320 | en |
| dc.identifier.uri | https://hdl.handle.net/10919/118953 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | Bartonella | en |
| dc.subject | small mammals | en |
| dc.subject | genetic diversity | en |
| dc.subject | evolution | en |
| dc.subject | tissue tropism | en |
| dc.title | Molecular Prevalence, Genetic Diversity, and Tissue Tropism of Bartonella Species in Small Mammals from Yunnan Province, China | en |
| dc.title.serial | Animals | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |