Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms

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dc.contributor.authorNooranidoost, Mohammaden
dc.contributor.authorCogan, N. G.en
dc.contributor.authorStoodley, Paulen
dc.contributor.authorGloag, Erin S.en
dc.contributor.authorHussaini, M. Yousuffen
dc.date.accessioned2024-01-09T15:59:30Zen
dc.date.available2024-01-09T15:59:30Zen
dc.date.issued2023-06-03en
dc.description.abstract<i>Pseudomonas aeruginosa</i> biofilms are relevant for a variety of disease settings, including pulmonary infections in people with cystic fibrosis. Biofilms are initiated by individual bacteria that undergo a phenotypic switch and produce an extracellular polymeric slime (EPS). However, the viscoelastic characteristics of biofilms at different stages of formation and the contributions of different EPS constituents have not been fully explored. For this purpose, we develop and parameterize a mathematical model to study the rheological behavior of three biofilms — P. aeruginosa wild type PAO1, isogenic rugose small colony variant (RSCV), and mucoid variant biofilms against a range of experimental data. Using Bayesian inference to estimate these viscoelastic properties, we quantify the rheological characteristics of the biofilm EPS. We employ a Monte Carlo Markov Chain algorithm to estimate these properties of P. aeruginosa variant biofilms in comparison to those of wild type. This information helps us understand the rheological behavior of biofilms at different stages of their development. The mechanical properties of wild type biofilms change significantly over time and are more sensitive to small changes in their composition than the other two mutants.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 100133 (Article number)en
dc.identifier.doihttps://doi.org/10.1016/j.bioflm.2023.100133en
dc.identifier.eissn2590-2075en
dc.identifier.issn2590-2075en
dc.identifier.orcidGloag, Erin [0000-0001-8895-3444]en
dc.identifier.otherPMC10313507en
dc.identifier.otherS2590-2075(23)00030-8 (PII)en
dc.identifier.pmid37396464en
dc.identifier.urihttps://hdl.handle.net/10919/117323en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37396464en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectExtracellular polymeric slimeen
dc.subjectBayesian estimationen
dc.subjectData Assimilationen
dc.subjectModelsen
dc.subjectFlowen
dc.subjectMatrixen
dc.titleBayesian estimation of <i>Pseudomonas aeruginosa</i> viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilmsen
dc.title.serialBiofilmen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2023-05-31en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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