Stac3 Is a Novel Regulator of Skeletal Muscle Development in Mice

dc.contributor.authorReinholt, Brad M.en
dc.contributor.authorGe, Xiaomeien
dc.contributor.authorCong, Xiaofeien
dc.contributor.authorGerrard, David E.en
dc.contributor.authorJiang, Honglinen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2017-03-06T20:32:02Zen
dc.date.available2017-03-06T20:32:02Zen
dc.date.issued2013-04-23en
dc.description.abstractThe goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice.en
dc.description.versionPublished versionen
dc.format.extent? - ? (8) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0062760en
dc.identifier.issn1932-6203en
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/75291en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318008400218&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsh3 domainsen
dc.subjectmyogenic differentiationen
dc.subjectmyoblast fusionen
dc.subjectgene-functionen
dc.subjectgenome-wideen
dc.subjectexpressionen
dc.subjectdrosophilaen
dc.subjectdistincten
dc.subjectmyf-5en
dc.subjectmyoden
dc.titleStac3 Is a Novel Regulator of Skeletal Muscle Development in Miceen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Stac3 is a novel regulator of skeletal muscle development in mice.pdf
Size:
904.64 KB
Format:
Adobe Portable Document Format
Description:
Publisher's Version
License bundle
Now showing 1 - 1 of 1
Name:
VTUL_Distribution_License_2016_05_09.pdf
Size:
18.09 KB
Format:
Adobe Portable Document Format
Description: