Intracellular invasion and survival of Brucella neotomae, another possible zoonotic Brucella species

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dc.contributor.authorWaldrop, Steven Granten
dc.contributor.authorSriranganathan, Nammalwaren
dc.date.accessioned2019-05-30T14:34:27Zen
dc.date.available2019-05-30T14:34:27Zen
dc.date.issued2019en
dc.description.abstractIn 1967, Brucella neotomae was first isolated from Neotoma lepida, the dessert wood rat, in Utah. With little infection data since its discovery, the zoonotic potential of this Brucella species is largely unknown. Recent reports of isolation from human cerebrospinal fluid, along with current literature suggest that B. neotomae has the ability to infect various hosts and cell types. In this report we extend the knowledge of B. neotomae ATCC 23459's intracellular invasion and survival abilities to a variety of cell lines through gentamicin protection assays. Some of the phagocytic and epithelial cell lines from various mammalian species represent characteristics of some cell types that could be encountered by Brucella in potential hosts. It was found that B. neotomae ATCC 23459 exhibits generally lower intracellular bacterial CFUs compared to the mouse-passaged strain of B. neotomae ATCC 23459, B. suis 1330, and B. abortus 2308. Ultimately, these observations provide a small piece of the puzzle in the investigation of the breadth of B. neotomae's pathogenic potential.en
dc.description.notesThe authors are supported by the Virginia-Maryland College of Veterinary Medicine's Research and Graduate Studies department. Support for this work was provided by the USDA and Smithfield. We thank Smithfield Foods Murphy Brown, LLC. (Virginia Tech Foundation, Inc. Fund #444115) and Virginia APHIS-USDA (Fund #422319) for their support. Additionally, the Virginia Tech's Open Access Subvention Fund covered the article processing charge. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.sponsorshipVirginia-Maryland College of Veterinary Medicine's Research and Graduate Studies department; USDA; Smithfield; Smithfield Foods Murphy Brown, LLC. (Virginia Tech Foundation, Inc.) [444115]; Virginia APHIS-USDA [422319]; Virginia Tech's Open Access Subvention Funden
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0213601en
dc.identifier.eissn1932-6203en
dc.identifier.issue4en
dc.identifier.othere0213601en
dc.identifier.pmid30943213en
dc.identifier.urihttp://hdl.handle.net/10919/89637en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIntracellular invasion and survival of Brucella neotomae, another possible zoonotic Brucella speciesen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Scholarly Works, Biomedical Sciences and Pathobiology
Destination Area: Global Systems Science (GSS)
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