Developmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in Bovine Liver

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dc.contributor.authorEleswarapu, Satyanarayana Venkataen
dc.contributor.committeechairJiang, Honglinen
dc.contributor.committeememberWong, Eric A.en
dc.contributor.committeememberDenbow, D. Michaelen
dc.contributor.committeememberTu, Zhijian Jakeen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2014-03-14T21:36:38Zen
dc.date.adate2004-06-22en
dc.date.available2014-03-14T21:36:38Zen
dc.date.issued2004-05-11en
dc.date.rdate2004-06-22en
dc.date.sdate2004-05-27en
dc.description.abstractLiver gene expression changes during development and is affected by growth hormone (GH). These changes in gene expression may be due to the differential expression of the liver-enriched transcription factors (LETFs). To study the potential involvement of LETFs in the regulation of gene expression in the bovine liver, we cloned the cDNA fragments of nine bovine LETFs, including hepatocyte nuclear factor (HNF)-1Æ Ã , 1Æ Ã , 3Æ Ã , 3Æ Ã , 3Æ Ã , 6, albumin D-element binding protein (DBP), and CCAAT/enhancer-binding proteins (C/EBP) -Æ Ã and Æ Ã , and compared the expression levels of them between adult and fetal bovine liver and between GH-treated and untreated adult bovine liver. The mRNA abundance of the LETFs was determined by ribonuclease protection assay (RPA). The cloned bovine LETF cDNA sequences showed high degrees of similarity (79 % to 99 %) to the LETF sequences of other species. The mRNA levels of HNF-1Æ Ã , HNF-3Æ Ã , and HNF-6 were significantly higher (P < 0.05) in the fetal liver (n=3) than in the adult liver (n=7). There were significant increases (P < 0.05) in the mRNA expression of HNF-3Æ Ã and HNF-6 in the liver of cows 24 h (n=6) and 1w (n=6) after GH administration. The results of this study suggest that HNF-1Æ Ã , HNF-3Æ Ã , and HNF-6 may play a role in differential regulation of gene expression between the fetal and adult bovine liver and that HNF-3Æ Ã and HNF-6 may be also involved in GH regulation of gene expression in the bovine liver.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05272004-100413en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05272004-100413/en
dc.identifier.urihttp://hdl.handle.net/10919/42801en
dc.publisherVirginia Techen
dc.relation.haspartThesisFinal.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectGrowth Hormoneen
dc.subjectBovineen
dc.subjectLiveren
dc.subjectLiver-Enriched Transcription Factorsen
dc.titleDevelopmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in Bovine Liveren
dc.typeThesisen
thesis.degree.disciplineAnimal and Poultry Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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