Antigenic Characterization of Haemophilus somnus Lipooligosaccharide

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dc.contributor.authorHoward, Michael D.en
dc.contributor.committeechairInzana, Thomas J.en
dc.contributor.committeememberSchurig, Gerhardt G.en
dc.contributor.committeememberNagarkatti, Mitzien
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:46:39Zen
dc.date.adate1998-11-16en
dc.date.available2014-03-14T20:46:39Zen
dc.date.issued1998-10-23en
dc.date.rdate1999-11-16en
dc.date.sdate1998-10-14en
dc.description.abstractLipooligosaccharide (LOS) is the major outer membrane component of many Gram-negative bacteria inhabiting the mucosal membranes, including pathogenic species of <I>Haemophilus</I> and <I>Neisseria</I>. LOS phase variation is one mechanism by which some of these bacteria avoid the host immune response. To better understand LOS phase variation as a virulence mechanism of <I>H. somnus</I>, knowledge of the antigenic diversity of LOS epitopes must be increased. Monoclonal antibodies (MAbs) to <I>H. somnus</I> LOS were produced and used with cross-reacting MAbs to <I>H. aegyptius</I> LOS (MAb 5F5) and <I>Neisseria</I> <I>gonorrhoeae</I> LOS (MAb 3F11) in an ELISA to investigate LOS heterogeneity among forty-five strains of <I>H. somnus</I>. Using three MAbs, thirty-nine of these <I>H. somnus</I> strains were grouped into six antigenic types. Three groups, associated solely with the cross-reacting MAbs 5F5 and 3F11, included the majority (76%) of <I>H. somnus</I> strains. The anti-<I>H. somnus</I> LOS MAb 5D7 recognized a low frequency epitope associated with each of the remaining three groups, which included 11% of the <I>H. somnus</I> strains. Six strains (13%) were not recognized by any of these MAbs. Inhibition ELISA experiments showed that the MAb 5F5 epitope contained phosphocholine (PCho) and this epitope was present in 56% of the strains tested. The MAb 5F5 epitope is phase variable in <I>H. somnus</I> LOS. How PCho negative variants could allow for systemic infection after initial colonization of the mucosa by PCho positive variants is discussed.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-101398-081516en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-101398-081516/en
dc.identifier.urihttp://hdl.handle.net/10919/35378en
dc.publisherVirginia Techen
dc.relation.haspartetd.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectLOSen
dc.subjectphase variationen
dc.subjecttyping schemeen
dc.subjectepitopeen
dc.subjectmonoclonal antibodyen
dc.titleAntigenic Characterization of <I>Haemophilus somnus</I> Lipooligosaccharideen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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