Characterization of the interaction of putrescine and the adenosine-3' ,5'-cyclic monophosphate-cAMP receptor protein complex in the regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli

Simple item page
dc.contributor.authorBusse, Leigh Anneen
dc.contributor.committeechairBoyle, Stephen M.en
dc.contributor.committeememberFalkinham, Joseph O. IIIen
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T21:33:36Zen
dc.date.adate2010-04-12en
dc.date.available2014-03-14T21:33:36Zen
dc.date.issued1988-04-07en
dc.date.rdate2010-04-12en
dc.date.sdate2010-04-12en
dc.description.abstractOrnithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine to produce the diamine, putrescine, in the bacterium Escherichia coli. The speC gene encoding ODC has been shown to be subject to transcriptional repression by either putrescine or the cAMP- cAMP receptor protein (CRP) complex. To determine whether these regulatory modes are independent, the expression of ODC was determined by measuring the specific activity of ODC in crude extracts prepared from exponentially grown cultures of wild type ~ coli K-12 as well as in strains unable to synthesize cAMP (cya) and/or CRP (crp). 1-5 mM cAMP repressed ODe activity 22-38% in wild type, 57-66% in the cya strain, and only 7-18% in the ~ strains. 2-10 mM putrescine repressed ODe activity 30-32% in wild type, 48-49% in the cya strain, and 37-38% in the ~ strain. As putrescine repressed ODe activity in the absence of eRP protein (i.e. in a crp strain), putrescine-mediated repression of ODe appears to be independent of the repression of ODC by the cAMP-eRP complex. This conclusion was verified by demonstrating th t .oDC repression by putrescine and cAMP together was additive.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 61 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-04122010-083645en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04122010-083645/en
dc.identifier.urihttp://hdl.handle.net/10919/42041en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.B877.pdfen
dc.relation.isformatofOCLC# 18272037en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.B877en
dc.subject.lcshEscherichia colien
dc.subject.lcshPolyaminesen
dc.titleCharacterization of the interaction of putrescine and the adenosine-3' ,5'-cyclic monophosphate-cAMP receptor protein complex in the regulation of the speC gene encoding ornithine decarboxylase in Escherichia colien
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
LD5655.V855_1988.B877.pdf
Size:
1.95 MB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses