Characterization of methionine transport in bovine intestinal brush border membrane vesicles
| dc.contributor.author | Dahl, Geoffrey Eliot | en |
| dc.contributor.department | Dairy Science | en |
| dc.date.accessioned | 2021-10-26T20:10:25Z | en |
| dc.date.available | 2021-10-26T20:10:25Z | en |
| dc.date.issued | 1987 | en |
| dc.description.abstract | Characteristics of methionine uptake by brush border membrane vesicles of bovine small intestine were investigated. Alkaline phosphatase marked the brush border membrane fraction obtained through differential centrifugation followed by a sucrose gradient. This preparation yielded a 10-fold enrichment of activity over homogenate. Methionine uptake was found to be into an osmotically active space. A binding constant of 75.4 pmol/mg membrane protein was determined. A significant (p<.05) sodium stimulation of methionine uptake was observed. This indicated active (energy-dependent) transport in addition to the diffusive component of intravesicular mention accumulation. Decreasing the pH of buffer medium significantly (p<.05) depresses methionine transport. A K<sub>m</sub> = .114 mM and V<sub>MAX</sub> = 56.5 pmol/s/mg membrane protein were ascertained for methionine. | en |
| dc.description.degree | M.S. | en |
| dc.format.extent | vi, 47 leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/106167 | en |
| dc.language.iso | en | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 17368648 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V855 1987.D334 | en |
| dc.subject.lcsh | Brush border membrane | en |
| dc.subject.lcsh | Methionine | en |
| dc.subject.lcsh | Ruminants -- Nutrition | en |
| dc.title | Characterization of methionine transport in bovine intestinal brush border membrane vesicles | en |
| dc.type | Thesis | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Dairy Science | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | masters | en |
| thesis.degree.name | M.S. | en |
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