Differential pathogenesis of Usutu virus isolates in mice

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dc.contributor.authorKuchinsky, Sarah C.en
dc.contributor.authorHawks, Seth A.en
dc.contributor.authorMossel, Eric C.en
dc.contributor.authorCoutermarsh-Ott, Sherylen
dc.contributor.authorDuggal, Nisha K.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-03-02T19:02:01Zen
dc.date.available2021-03-02T19:02:01Zen
dc.date.issued2020-10-12en
dc.description.abstractUsutu virus (USUV; Flavivirus), a close phylogenetic and ecological relative of West Nile virus, is a zoonotic virus that can cause neuroinvasive disease in humans. USUV is maintained in an enzootic cycle between Culex mosquitoes and birds. Since the first isolation in 1959 in South Africa, USUV has spread throughout Africa and Europe. Reported human cases have increased over the last few decades, primarily in Europe, with symptoms ranging from mild febrile illness to severe neurological effects. In this study, we investigated whether USUV has become more pathogenic during emergence in Europe. Interferon α/β receptor knockout (Ifnar1-/-) mice were inoculated with recent USUV isolates from Africa and Europe, as well as the historic 1959 South African strain. The three tested African strains and one European strain from Spain caused 100% mortality in inoculated mice, with similar survival times and histopathology in tissues. Unexpectedly, a European strain from the Netherlands caused only 12% mortality and significantly less histopathology in tissues from mice compared to mice inoculated with the other strains. Viremia was highest in mice inoculated with the recent African strains and lowest in mice inoculated with the Netherlands strain. Based on phylogenetics, the USUV isolates from Spain and the Netherlands were derived from separate introductions into Europe, suggesting that disease outcomes may differ for USUV strains circulating in Europe. These results also suggest that while more human USUV disease cases have been reported in Europe recently, circulating African USUV strains are still a potential major health concern.en
dc.description.sponsorshipFunding was received from the Virginia- Maryland College of Veterinary Medicine (NKD). USUV isolate TMNetherlands was received from the European Virus Archive goes Global (EVAg) project that has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 653316.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKuchinsky SC, Hawks SA, Mossel EC, Coutermarsh-Ott S, Duggal NK (2020) Differential pathogenesis of Usutu virus isolates in mice. PLoS Negl Trop Dis 14(10): e0008765. https://doi.org/ 10.1371/journal.pntd.0008765en
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0008765en
dc.identifier.issue10en
dc.identifier.urihttp://hdl.handle.net/10919/102520en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.titleDifferential pathogenesis of Usutu virus isolates in miceen
dc.title.serialPlos Neglected Tropical Diseasesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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