The effect of uterine flushings on early bovine embryo development in vitro

dc.contributor.authorToole, Robert J.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2019-03-26T19:16:58Zen
dc.date.available2019-03-26T19:16:58Zen
dc.date.issued1984en
dc.description.abstractBovine morulae (day 6; n=328) were obtained to evaluate the effect of uterine flushings (UF) obtained from ovariectomized progesterone (P) or estrogen (E) treated cows or from previously superovulated cows on day 6, 10, or 15 post insemination on early embryo development. Ninety-eight cows were superovulated with follicle stimulating hormone. Embryos were collected by non-surgical flushing procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in Ham's F-10 containing 10% steer serum (SS), 10% P or E concentrated UF, 10% SS plus 1% UF from day 6, 10, or 15, or different concentrations of UF from day 6, 10, or 15 (10%, 1%, .1%, .01%). Embryos were cultured up to the hatched blastocyst stage or degeneration and evaluated at 12 h intervals. Treatment differences were evaluated by analysis of variance after assigning a value of 0 to 5 to each embryo representing its final stage of development. Overall analysis revealed that embryo development was significantly affected by supplement, but not affected by initial quality. Final developmental score was significantly higher in SS than in all other supplements. Combination of 1% UF and 10% SS resulted in enhanced embryo development compared to all single UF protein treatments. Time to the hatching blastocyst and hatched blastocyst developmental stages was reduced (P<.05) in 1% day 15 UF compared to 1% day 6 and day 10 UF plus 10% SS, or 10% SS. These results suggest that day 15 UF may contain a substance which may shorten the time of embryo hatching in vitro.en
dc.description.degreeMaster of Scienceen
dc.format.extentxi, 160 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/88582en
dc.language.isoen_USen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 11645311en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1984.T664en
dc.subject.lcshCattle -- Reproductionen
dc.titleThe effect of uterine flushings on early bovine embryo development in vitroen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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