The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro

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dc.contributor.authorAnkrapp, David P.en
dc.contributor.committeechairBevan, David R.en
dc.contributor.committeememberEbel, Richard E.en
dc.contributor.committeememberSitz, Thomas O.en
dc.contributor.committeememberPotts, Malcolmen
dc.contributor.committeememberVeit, Hugo P.en
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:20:45Zen
dc.date.adate2005-10-10en
dc.date.available2014-03-14T21:20:45Zen
dc.date.issued1993-05-05en
dc.date.rdate2005-10-10en
dc.date.sdate2005-10-10en
dc.description.abstractThe concentration of insulin-like growth factor I (IGF-I) in tissue taken from human non-small cell lung carcinoma (NSCLC) is 1.4- to 7-fold higher than the concentration of IGF-I in the surrounding normal lung tissue and therefore IGF-I may be involved in the growth of NSCLC. In this study it was determined that NSCLC cell lines (A549, A427, SK-LU-1) expressed the type I IGF-I receptor protein and IGF-I stimulated the proliferation of low density plated (2000 cells/cm² growth area) carcinoma cells by 1.6- to 3- fold above control after a four day inCUbation period under serum-free conditions (A549, A427) or in the presence of 0.25% serum (SK-LU-1). In addition, when added to detergent-solubilized type I IGF receptors from A549 cells, IGF-I stimulated [1] a dose-dependent increase in the autophosphorylation of the type I IGF receptor, and [2] a dose-dependent increase (1.5- to 4-fold) in the phosphorylation of a tyrosine kinase-specific substrate. These results suggest that the growth promoting activity of IGF-I for the lung carcinoma cells was mediated through the activation of the type I IGF receptor.en
dc.description.degreePh. D.en
dc.format.extentxiii, 134 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10102005-131608en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10102005-131608/en
dc.identifier.urihttp://hdl.handle.net/10919/39726en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1993.A557.pdfen
dc.relation.isformatofOCLC# 28732754en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1993.A557en
dc.subject.lcshCancer cells -- Growthen
dc.subject.lcshLungs -- Canceren
dc.subject.lcshSomatomedinen
dc.titleThe effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitroen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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