Detection of Hammondia heydorni-like organisms and their differentiation from Neospora caninum using random-amplified polymorphic DNA-polymerase chain reaction

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dc.contributor.authorSreekumar, C.en
dc.contributor.authorHill, Dolores E.en
dc.contributor.authorFournet, V. M.en
dc.contributor.authorRosenthal, B. M.en
dc.contributor.authorLindsay, David S.en
dc.contributor.authorDubey, Jitender P.en
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:11Zen
dc.date.available2014-06-20T14:13:11Zen
dc.date.issued2003-10en
dc.description.abstractNeospora caninum and Hammondia heydorni are morphologically and phylogenetically related coccidians that are found in dogs. New diagnostic genetic loci, based on random-amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) were developed to aid in the detection of H. heydorni like parasites and to discriminate them from N. caninum and other related coccidians of dogs. On the basis of the data obtained from 5 random decamers, H. heydorni (Manhattan-1) and N. caninum (NC1) were characterized by distinct banding patterns (similarity index = 0.068). High-stringency PCR assays were developed from the sequences of 2 cloned hands (GenBank BZ592549 and BZ592593), uniquely amplified from H. heydorni. Interestingly, using these primers, PCR amplification was achieved only from 2 of the 5 isolates presumed to represent H. heydorni. The same result was obtained From these 5 isolates using a recently described PCR assay directed to the H. heydorni internal transcribed spacer-1. It is concluded that H. heydorni and N. caninum are genetically distinct and that such tools may be useful for more detailed characterization of the diversity of related parasites occurring in dogs.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationC. Sreekumar, D. E. Hill, V. M. Fournet, B. M. Rosenthal, D. S. Lindsay, and J. P. Dubey (2003). "Detection of Hammondia heydorni-like Organisms and Their Differentiation From Neospora caninum Using Random-Amplified Polymorphic DNA-polymerase Chain Reaction," Journal of Parasitology, Vol. 89, No. 5, pp. 1082-1085. doi: http://dx.doi.org/10.1645/GE-93Ren
dc.identifier.doihttps://doi.org/10.1645/ge-93ren
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49034en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-93Ren
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectarbitrary primersen
dc.subjectribosomal-rnaen
dc.subjectpcren
dc.subjectdogsen
dc.subjectoocystsen
dc.subjectidentificationen
dc.subjectamplificationen
dc.subjectdiagnosisen
dc.subjectparasitologyen
dc.titleDetection of Hammondia heydorni-like organisms and their differentiation from Neospora caninum using random-amplified polymorphic DNA-polymerase chain reactionen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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