dc.contributor.author	Das, Swati Jr.	en
dc.contributor.committeechair	Berry, Duane F.	en
dc.contributor.committeemember	Zelazny, Lucian W.	en
dc.contributor.committeemember	Love, Nancy G.	en
dc.contributor.department	Crop and Soil Environmental Sciences	en
dc.date.accessioned	2014-03-14T20:50:56Z	en
dc.date.adate	1998-02-26	en
dc.date.available	2014-03-14T20:50:56Z	en
dc.date.issued	1997-02-12	en
dc.date.rdate	1999-02-26	en
dc.date.sdate	1997-02-12	en
dc.description.abstract	This feasibility study was performed at Virginia Polytechnic Institute and State University (Blacksburg, VA) in collaboration with BioSystems Technology, Inc. (Blacksburg, VA). In this study, degradability of PCBs (Aroclor 1242) from an aged surface soil was evaluated using serum bottle microcosms containing aceticlastic methanogenic consortium, enriched from a municipal anaerobic digester. Two different experiments, "intermediate feed" and "starve and feed" were conducted by manipulating the methanogenic consortium with different amounts of acetate feeding, during 30 days of incubation. Disappearance of Aroclor 1242 in the microcosms was quantified using gas chromatography (GC). Significant differences in Aroclor 1242 removal between inoculated and uninoculated (control) microcosms were observed suggesting that the methanogenic consortium was responsible for Aroclor 1242 disappearance. However, GC-mass spectrometry (GC-MS) results could not confirm that disappearance of Aroclor 1242 was due to anaerobic dehalogenation. From another experiment, it was confirmed that removal of Aroclor 1242 was not due to evaporation losses during sample extraction. Toxicity of an aged Aroclor 1242 contaminated surface soil was evaluated on an aceticlastic methanogenic consortium, enriched from a municipal anaerobic digester. Microcosms were set up using different amounts of soil and inoculum. Total gas production in the microcosms was monitored during 30 days of incubation, using a glass syringe. Total methane production in the microcosms was quantitated using GC. Toxicity of the soil on the methanogenic inoculum was determined based on the decreased rate of methane production in the microcosms relative to non- soil containing controls. Compared to the control, there was reduction in total methane production in soil containing microcosms. Between 3-27% reduction in total methane production was noticed in microcosms containing different amounts of soil and consortium. Reduction in methane production seemed to increase with increasing amount of soil. Whether this decrease in methane production was due to toxicity of Aroclor 1242 on the methanogenic consortium or due possibly to the toxicity of trapped oxygen in the soil could not be determined. The rate of gas production in the soil microcosm was linear.	en
dc.description.degree	Master of Science	en
dc.identifier.other	etd-12498-13011	en
dc.identifier.sourceurl	http://scholar.lib.vt.edu/theses/available/etd-12498-13011/	en
dc.identifier.uri	http://hdl.handle.net/10919/36501	en
dc.publisher	Virginia Tech	en
dc.relation.haspart	Swatietd.PDF	en
dc.relation.haspart	cpt1.pdf	en
dc.relation.haspart	cpt2.pdf	en
dc.relation.haspart	cpt3.pdf	en
dc.relation.haspart	cpt4.pdf	en
dc.relation.haspart	cpt5.pdf	en
dc.relation.haspart	cpt6.pdf	en
dc.rights	In Copyright	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	en
dc.subject	PCBs	en
dc.subject	bioremediation	en
dc.subject	contaminated surface soil	en
dc.subject	methanogenic consortium.	en
dc.title	Bioremediation of Pcb Contaminated Surface Soil: A Microcosm Study	en
dc.type	Thesis	en
thesis.degree.discipline	Crop and Soil Environmental Sciences	en
thesis.degree.grantor	Virginia Polytechnic Institute and State University	en
thesis.degree.level	masters	en
thesis.degree.name	Master of Science	en

Bioremediation of Pcb Contaminated Surface Soil: A Microcosm Study

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