Growth enhancement and selection attempts for Spirillum volutans
| dc.contributor.author | Friedman, Michael W. | en |
| dc.contributor.department | Microbiology | en |
| dc.date.accessioned | 2021-10-26T20:10:30Z | en |
| dc.date.available | 2021-10-26T20:10:30Z | en |
| dc.date.issued | 1987 | en |
| dc.description.abstract | Project objectives were to develop an improved plating medium to achieve reproducible colony counts and accurate quantification for growth or survival of Spirillum volutans; and to develop an improved method for selection of S. volutans from nature. Growth enhancement studies began with casein hydrolysate-succinate-salts medium (CHSS). Casein hydrolysate concentration, type of casein hydrolysate, pH, and phosphate concentration optima were determined. Growth of S. volutans doubled after 24 h with the addition of potassium phosphate buffer (1 mM final concentration) to CHSS medium (CHSS-P). Addition of supplements to CHSS-P medium, and modified Bordetella and Brucella media failed to increase the cell yields of S. volutans. Punctiform colonies did form in CHSS-P medium (0.7% agar) pour-plates, yet at a recovery rate much lower than reported by Padgett et at. (1982) for surface growth on spread plates. The role of amino compounds as possible growth factors was investigated. Thin-layer chromatography, gas chromatography, and amino acid analysis were employed to identify those amino compounds (if any) that decreased or disappeared in the medium after 48 h of growth. None could be found. Numbers of viable cells were not greatly increased, however, viability was prolonged by growing S. volutans in dialysis sacs suspended in CHSS-P medium. The tolerances of S. volutans to several antimicrobial compounds were determined. Attempts at using hay infusion, Pringsheim, and CHSS-P media supplemented with various compounds as selective agents did not result in predominance of S. volutans. | en |
| dc.description.degree | M.S. | en |
| dc.format.extent | xiii, 106 leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/106185 | en |
| dc.language.iso | en | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 17374758 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V855 1987.F743 | en |
| dc.subject.lcsh | Campylobacter | en |
| dc.subject.lcsh | Spirillaceae | en |
| dc.title | Growth enhancement and selection attempts for Spirillum volutans | en |
| dc.type | Thesis | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Microbiology | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | masters | en |
| thesis.degree.name | M.S. | en |
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