Analysis of Ethoxyquin and its Oxidation Products using Supercritical Fluid Extraction and High Performance Liquid Chromatography with Chemiluminescent Nitrogen Detection

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dc.contributor.authorBrannegan, Daniel Roberten
dc.contributor.committeechairTaylor, Larry T.en
dc.contributor.committeememberGlanville, James O.en
dc.contributor.committeememberMcNair, Harold M.en
dc.contributor.departmentChemistryen
dc.date.accessioned2014-03-14T20:32:58Zen
dc.date.adate2000-03-31en
dc.date.available2014-03-14T20:32:58Zen
dc.date.issued2000-03-10en
dc.date.rdate0000-00-00en
dc.date.sdate2000-03-30en
dc.description.abstractEthoxyquin is an antioxidant commonly used to preserve vitamins and lipids in various food products and animal feeds. The extraction and determination of ethoxyquin is becoming increasingly important as products, which are labeled as "natural" are becoming more common. The present method of determination only ensures that ethoxyquin values are below 10-20 parts per million. Therefore, advances are needed in methods of extraction and analysis in order to lower the detection limits in various products. The first part of this research investigates the use of supercritical fluids in the extraction of ethoxyquin from lean beef and beef fat. Supercritical fluids offer the advantages of safety, time, expense, and selectivity over liquid extractions. Three fluids were examined: carbon dioxide, trifluoromethane, and 1,1,1,2-tetrafluoroethane. Carbon dioxide appeared to react with ethoxyquin during the extraction. Methanol modified hydrofluorocarbons provided more complete extractions over pure hydrofluorocarbon fluids. Methanol modified 1,1,1,2-tetrafluoroethane was used in the extraction of ethoxyquin from lean beef and beef fat, and provided a quantitative extraction at the 0.5 ppm level. The second part of this research centered on the separation and quantitation of the oxidation products of ethoxyquin through the use of high pressure liquid chromatography with chemiluminescence nitrogen detection (HPLC/CLND). When ethoxyquin is oxidized, the resulting products also exhibit antioxidative properties. While these oxidation products are known, no effort has been made to separate and quantify them in real or clean samples. HPLC/CLND allows all nitrogen containing compounds to be quantified without a known standard. This method is of extreme interest in the case of ethoxyquin oxidation products, or other types of metabolites, where standards are difficult to obtain or are unstable. HPLC/CLND allowed a separation of ethoxyquin and four of its oxidation products to be detected, thus making future studies of the antioxidant behavior of ethoxyquin feasible.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-03302000-20440044en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03302000-20440044/en
dc.identifier.urihttp://hdl.handle.net/10919/31575en
dc.publisherVirginia Techen
dc.relation.haspartTheses316_doc.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectOxidationen
dc.subjectSFCen
dc.subjectExtractionen
dc.subjectEthoxyquinen
dc.subjectChromatographyen
dc.subjectGC/MSen
dc.subjectSupercriticalen
dc.subjectHPLC/CLNDen
dc.subjectBeefen
dc.subjectHPLCen
dc.subjectBeef Faten
dc.titleAnalysis of Ethoxyquin and its Oxidation Products using Supercritical Fluid Extraction and High Performance Liquid Chromatography with Chemiluminescent Nitrogen Detectionen
dc.typeThesisen
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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