A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications
TR Number
Date
2015-03-01
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
Description
Keywords
Cell Biology, autophagy, autophagy flux, autophagy response, Cyto-ID, RNA interference screen, small molecule screen, spectrophotometric assay, 3-MA, 3-methyladenine, FBS, fetal bovine serum, GFP, green fluorescent protein, LAMP1, lysosomal-associated membrane protein 1, MAP1LC3B, LC3B, microtubule-associated protein 1 light chain 3 beta, MAP3K6, mitogen-activated protein kinase kinase kinase 6, MDC, monodansylcadaverine, mRFP, monomeric red fluorescent protein, MTOR, mechanistic target of rapamycin, NS, nonsilencing, RAB5A, member RAS oncogene family, RNAi, RNA interference, shRNA, short-hairpin RNA, SQSTM1, sequestosome 1, WNK2, WNK lysine deficient protein kinase 2, SIRNA SCREEN, LIVING CELLS, TRANSCRIPTION, CHLOROQUINE, KINASES, DISEASE, MARKER, TRIAL, LC3