Photo-Disassembly of Membrane Microdomains Revives Conventional Antibiotics against MRSA

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dc.contributor.authorHui, Jieen
dc.contributor.authorDong, Pu-Tingen
dc.contributor.authorLiang, Lijiaen
dc.contributor.authorMandal, Taraknathen
dc.contributor.authorLi, Junjieen
dc.contributor.authorUlloa, Erlinda R.en
dc.contributor.authorZhan, Yueweien
dc.contributor.authorJusuf, Sebastianen
dc.contributor.authorZong, Chengen
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorLiu, George Y.en
dc.contributor.authorCui, Qiangen
dc.contributor.authorCheng, Ji-Xinen
dc.date.accessioned2020-09-21T16:14:15Zen
dc.date.available2020-09-21T16:14:15Zen
dc.date.issued2020-03-01en
dc.date.updated2020-09-21T16:14:13Zen
dc.description.abstractConfronted with the rapid evolution and dissemination of antibiotic resistance, there is an urgent need to develop alternative treatment strategies for drug-resistant pathogens. Here, an unconventional approach is presented to restore the susceptibility of methicillin-resistant S. aureus (MRSA) to a broad spectrum of conventional antibiotics via photo-disassembly of functional membrane microdomains. The photo-disassembly of microdomains is based on effective photolysis of staphyloxanthin, the golden carotenoid pigment that gives its name. Upon pulsed laser treatment, cell membranes are found severely disorganized and malfunctioned to defense antibiotics, as unveiled by membrane permeabilization, membrane fluidification, and detachment of membrane protein, PBP2a. Consequently, the photolysis approach increases susceptibility and inhibits development of resistance to a broad spectrum of antibiotics including penicillins, quinolones, tetracyclines, aminoglycosides, lipopeptides, and oxazolidinones. The synergistic therapy, without phototoxicity to the host, is effective in combating MRSA both in vitro and in vivo in a mice skin infection model. Collectively, this endogenous chromophore-targeted phototherapy concept paves a novel platform to revive conventional antibiotics to combat drug-resistant S. aureus infections as well as to screen new lead compounds.en
dc.description.versionPublished versionen
dc.format.extent13 page(s)en
dc.format.mediumElectronic-eCollectionen
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 1903117 (Article number)en
dc.identifier.doihttps://doi.org/10.1002/advs.201903117en
dc.identifier.eissn2198-3844en
dc.identifier.issn2198-3844en
dc.identifier.issue6en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.otherADVS1556 (PII)en
dc.identifier.pmid32195102 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100031en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherWileyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectChemistry, Multidisciplinaryen
dc.subjectNanoscience & Nanotechnologyen
dc.subjectMaterials Science, Multidisciplinaryen
dc.subjectChemistryen
dc.subjectMaterials Scienceen
dc.subjectantibiotic resistanceen
dc.subjectmembrane microdomainsen
dc.subjectpulsed lasersen
dc.subjectStaphylococcus aureusen
dc.subjectstaphyloxanthinen
dc.subjectRESISTANT STAPHYLOCOCCUS-AUREUSen
dc.subjectPIGMENTen
dc.subjectDAPTOMYCINen
dc.subjectMECHANISMSen
dc.subjectVIRULENCEen
dc.subjectCHARGEen
dc.titlePhoto-Disassembly of Membrane Microdomains Revives Conventional Antibiotics against MRSAen
dc.title.serialAdvanced Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen

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