In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines

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dc.contributor.authorFilep, Reneeen
dc.contributor.committeechairAkers, Robert Michaelen
dc.contributor.committeememberPearson, Ronald E.en
dc.contributor.committeememberSaacke, Richard G.en
dc.contributor.committeememberVinson, William E.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T21:37:31Zen
dc.date.adate2009-06-10en
dc.date.available2014-03-14T21:37:31Zen
dc.date.issued1990-02-05en
dc.date.rdate2009-06-10en
dc.date.sdate2009-06-10en
dc.description.abstractThe ability of mammary tissue from mature Holstein bulls to respond to hormonal stimuli in organ culture to synthesize and secrete milk proteins was studied. Sixteen bulls from the genetic selection and control lines developed at Virginia Polyteclmic Institute were randomly assigned to either a non-treated or steroid pretreated group. The steroid pretreated group received estradiol 17-B and progesterone at 0.1 and 0.25 mg/kg/day, respectively, for 7 d starting 15 d before slaughter. Mammary tissue was explanted and cultured for up to 96 h in basal medium (B) which consisted of Media 199 supplemented with fetal calf serum and bovine insulin, or stimulatory medium (St), which was further supplemented with triiodothyronine, estradiol 17-B, hydrocortisone, and bovine prolactin (PRL). Explants in duplicate multi-well culture dishes were incubated in the presence of 3H-Iabeled amino acids or 3H-Iabeled thymidine. Measurements made included: casein in media and homogenized tissue, alpha-lactalbumin in homogenized tissue, tri-chloro-acetic acid precipitable 3H-protein in media and homogenized tissue, and DNA content of cultured tissue. A subset of cultured explants from each bull was fixed and embedded for histological evaluation and auto radiographic localization of incorporated 3H-labeled thymidine. Synthesis and secretion data, as well as growth responses of the epithelium were compared between selection and control bulls.en
dc.description.degreeMaster of Scienceen
dc.format.extentxiii, 141 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06102009-063451en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06102009-063451/en
dc.identifier.urihttp://hdl.handle.net/10919/43016en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1990.F644.pdfen
dc.relation.isformatofOCLC# 21767002en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1990.F644en
dc.subject.lcshHolstein-Friesian cattleen
dc.subject.lcshMilk yielden
dc.titleIn vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic linesen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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