Evidence for extensive heterotrophic metabolism, antioxidant action, and associated regulatory events during winter hardening in Sitka spruce

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dc.contributor.authorCollakova, Evaen
dc.contributor.authorKlumas, Curtisen
dc.contributor.authorSuren, Haktanen
dc.contributor.authorMyers, Elijahen
dc.contributor.authorHeath, Lenwood S.en
dc.contributor.authorHolliday, Jason A.en
dc.contributor.authorGrene, Ruthen
dc.contributor.departmentComputer Scienceen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2013-05-10T15:10:41Zen
dc.date.available2013-05-10T15:10:41Zen
dc.date.issued2013-04-30en
dc.date.updated2013-05-10T15:10:41Zen
dc.description.abstractBackground Cold acclimation in woody perennials is a metabolically intensive process, but coincides with environmental conditions that are not conducive to the generation of energy through photosynthesis. While the negative effects of low temperatures on the photosynthetic apparatus during winter have been well studied, less is known about how this is reflected at the level of gene and metabolite expression, nor how the plant generates primary metabolites needed for adaptive processes during autumn. Results The MapMan tool revealed enrichment of the expression of genes related to mitochondrial function, antioxidant and associated regulatory activity, while changes in metabolite levels over the time course were consistent with the gene expression patterns observed. Genes related to thylakoid function were down-regulated as expected, with the exception of plastid targeted specific antioxidant gene products such as thylakoid-bound ascorbate peroxidase, components of the reactive oxygen species scavenging cycle, and the plastid terminal oxidase. In contrast, the conventional and alternative mitochondrial electron transport chains, the tricarboxylic acid cycle, and redox-associated proteins providing reactive oxygen species scavenging generated by electron transport chains functioning at low temperatures were all active. Conclusions A regulatory mechanism linking thylakoid-bound ascorbate peroxidase action with “chloroplast dormancy” is proposed. Most importantly, the energy and substrates required for the substantial metabolic remodeling that is a hallmark of freezing acclimation could be provided by heterotrophic metabolism.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Plant Biology. 2013 Apr 30;13(1):72en
dc.identifier.doihttps://doi.org/10.1186/1471-2229-13-72en
dc.identifier.urihttp://hdl.handle.net/10919/22036en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderEva Collakova et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleEvidence for extensive heterotrophic metabolism, antioxidant action, and associated regulatory events during winter hardening in Sitka spruceen
dc.title.serialBMC Plant Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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