Comparison of florfenicol depletion in dairy goat milk using ultra-performance liquid chromatography with tandem mass spectrometry and a commercial on-farm test

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dc.contributor.authorRichards, Emily D.en
dc.contributor.authorPereira, Richard V.en
dc.contributor.authorDavis, Jennifer L.en
dc.contributor.authorRowe, Joan D.en
dc.contributor.authorClapham, Maaike O.en
dc.contributor.authorWetzlich, Scott E.en
dc.contributor.authorRupchis, Benjamin A.en
dc.contributor.authorTell, Lisa A.en
dc.date.accessioned2024-01-19T13:28:55Zen
dc.date.available2024-01-19T13:28:55Zen
dc.date.issued2022-08-29en
dc.description.abstractFlorfenicol is a broad-spectrum antibiotic commonly prescribed in an extra-label manner for treating meat and dairy goats. Scientific data in support of a milk withdrawal interval recommendation is limited to plasma pharmacokinetic data and minimal milk residue data that is limited to cattle. Therefore, a rapid residue detection test (RRDT) could be a useful resource to determine if milk samples are free of drug residues and acceptable for sale. This study compared a commercially available RRDT (Charm® FLT strips) to detect florfenicol residues in fresh milk samples from healthy adult dairy breed goats treated with florfenicol (40 mg/kg subcutaneously twice 4 days apart) with quantitative analysis of florfenicol concentrations using ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). In addition, storage claims for testing bovine milk using the RRDT were assessed using stored goat milk samples. Milk samples were collected every 12 h for a minimum of 26 days. Commercial RRDT strips remained positive in individual goats ranging from 528 to 792 h (22–33 days) after the second dose, whereas, UPLC-MS/MS indicated the last detectable florfenicol concentration in milk samples ranged from 504 to 720 h (21–30 days) after the second dose. Results from stored milk samples from treated goats indicate that samples can be stored for up to 5 days in the refrigerator and 60 days in the freezer after milking prior to being tested with a low risk of false-negative test results due to drug degradation. Elevated somatic cell counts and bacterial colony were noted in some of the milk samples in this study, but further study is required to understand the impact of these quality factors on RRDT results.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 991772 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fvets.2022.991772en
dc.identifier.eissn2297-1769en
dc.identifier.issn2297-1769en
dc.identifier.orcidDavis, Jennifer [0000-0002-7930-4589]en
dc.identifier.otherPMC9465015en
dc.identifier.pmid36105005en
dc.identifier.urihttps://hdl.handle.net/10919/117405en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36105005en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectflorfenicolen
dc.subjectgoaten
dc.subjectextra-label drug useen
dc.subjectdrug residueen
dc.subjectmilk residueen
dc.titleComparison of florfenicol depletion in dairy goat milk using ultra-performance liquid chromatography with tandem mass spectrometry and a commercial on-farm testen
dc.title.serialFrontiers in Veterinary Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2022-08-05en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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