Development of Gold Nanocluster-Based Biosensors

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dc.contributor.authorZhou, Xinzheen
dc.contributor.committeechairMarr, Linsey C.en
dc.contributor.committeememberPruden, Amyen
dc.contributor.committeememberVikesland, Peter J.en
dc.contributor.departmentCivil and Environmental Engineeringen
dc.date.accessioned2017-03-25T06:00:31Zen
dc.date.available2017-03-25T06:00:31Zen
dc.date.issued2015-10-01en
dc.description.abstractGold nanoclusters possess both theoretical and practical importance in the development of ultrasensitive biosensors based on surface-enhanced Raman spectroscopy (SERS). Manipulation of gold nanoclusters in a predictable and reproducible manner for the application of refined biochemical analysis still remains challenging. In this study, high-purity gold nanoclusters are isolated via a simple method based on density gradient centrifugation. Three distinct bands including monomers, small aggregates (2-4 nanospheres), and large aggregates (>5 nanospheres) can be separated via density gradient centrifugation. The isolated gold nanoclusters greatly enhance the Raman intensity of the trapped dye molecules such that single nanocluster detection is feasible. To develop a gold nanoparticle-based biosensor for influenza virus, effort was also made to modify recognition moieties such as aptamers to gold nanoparticles via distinct approaches. The increase of hydraulic diameter and the shift of optical absorbance spectrum indicate the success of surface modification to gold nanoparticles.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:6307en
dc.identifier.urihttp://hdl.handle.net/10919/76678en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectGold Nanoclustersen
dc.subjectDensity Gradient Centrifugationen
dc.subjectSurface-Enhanced Raman Scatteringen
dc.subjectInfluenza Virusen
dc.subjectAptameren
dc.subjectBiosensoren
dc.titleDevelopment of Gold Nanocluster-Based Biosensorsen
dc.typeThesisen
thesis.degree.disciplineEnvironmental Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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