Alkylation of rat lens crystallins with iodoacetamide

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dc.contributor.authorHaynes, Linda Roseen
dc.contributor.committeechairHess, John L.en
dc.contributor.committeememberBarnett, Lewis B.en
dc.contributor.committeememberBunce, George Edwinen
dc.contributor.committeememberRutherford, Charles L.en
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:41:26Zen
dc.date.adate2010-07-28en
dc.date.available2014-03-14T21:41:26Zen
dc.date.issued1977-09-05en
dc.date.rdate2010-07-28en
dc.date.sdate2010-07-28en
dc.description.abstractAlkylation of lens proteins with iodoacetamide during homogenization of tissue (50 millimolar excess) immediately followed by gel-permeation chromatography yielded a crystallin population devoid of βH-crystallin. This result occurred in lens homogenates from both young (100 g) and older (400 g) male rats. BetaH-crystallin was not converted to insoluble protein with alkylation. Each crystallin fraction reacted with radioactive iodoacetamide in proportion to sulfhydryl content; at a ratio of 1 mg iodoacetamide/mg protein total free-sulfhydryl of the crystallins had reacted after 1 hr at pH 8, 25°C. Alkylated α-, βL-' and y-crystallin fractions demonstrated no altered chromatographic behavior on Sephacryl S-200; only alkylated βH-crystallin was altered so that it co-chromatographed with control or alkylated βL-crystallin.en
dc.description.degreeMaster of Scienceen
dc.format.extent73 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07282010-020246en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07282010-020246/en
dc.identifier.urihttp://hdl.handle.net/10919/43951en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1977.H39.pdfen
dc.relation.isformatofOCLC# 21387698en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1977.H39en
dc.subject.lcshAlkylationen
dc.titleAlkylation of rat lens crystallins with iodoacetamideen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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