Pioneer colonizers: Bacteria that alter the chicken intestinal morphology and development of the microbiota

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dc.contributor.authorLee, Margie D.en
dc.contributor.authorPedroso, Adriana A.en
dc.contributor.authorLumpkins, Bretten
dc.contributor.authorCho, Youngjaeen
dc.contributor.authorMaurer, John J.en
dc.date.accessioned2023-09-26T13:44:53Zen
dc.date.available2023-09-26T13:44:53Zen
dc.date.issued2023-03en
dc.description.abstractMicrobes commonly administered to chickens facilitate development of a beneficial microbiome that improves gut function, feed conversion and reduces pathogen colonization. Competitive exclusion products, derived from the cecal contents of hens and shown to reduce Salmonella colonization in chicks, possess important pioneer-colonizing bacteria needed for proper intestinal development and animal growth. We hypothesized that inoculation of these pioneer-colonizing bacteria to day of hatch chicks would enhance the development of their intestinal anatomy and microbiome. A competitive exclusion product was administered to broiler chickens, in their drinking water, at day of hatch, and its impact on intestinal morphometrics, intestinal microbiome, and production parameters, was assessed relative to a control, no treatment group. 16S rRNA gene, terminal restriction fragment length polymorphism (T-RFLP) was used to assess ileal community composition. The competitive exclusion product, administered on day of hatch, increased villus height, villus height/width ratio and goblet cell production similar to 1.25-fold and expression of enterocyte sugar transporters 1.25 to 1.5-fold in chickens at 3 days of age, compared to the control group. As a next step, chicks were inoculated with a defined formulation, containing Bacteroidia and Clostridia representing pioneercolonizing bacteria of the two major bacterial phyla present in the competitive exclusion product. The defined formulation, containing both groups of bacteria, were shown, dependent on age, to improve villus height (jejunum: 1.14 to 1.46fold; ileum: 1.17-fold), goblet cell numbers (ileum 1.32 to 2.51-fold), and feed efficiency (1.18-fold, day 1) while decreasing Lactobacillus ileal abundance by onethird to half in birds at 16 and 42 days of age, respectively; compared to the phosphate buffered saline treatment group. Therefore, specific probiotic formulations containing pioneer colonizing species can provide benefits in intestinal development, feed efficiency and body weight gain.en
dc.description.notesThis work was supported by United States Department of Agriculture formula funds and the State of Georgia's Veterinary Medicine Agricultural Research Grant. JM was supported by USDA HATCH fund: VA-160130.en
dc.description.sponsorshipUnited States Department of Agriculture formula funds; State of Georgia's Veterinary Medicine Agricultural Research Grant; USDA HATCH fund [VA-160130]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphys.2023.1139321en
dc.identifier.eissn1664-042Xen
dc.identifier.other1139321en
dc.identifier.pmid37064908en
dc.identifier.urihttp://hdl.handle.net/10919/116335en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmicrobiomeen
dc.subjectperformanceen
dc.subjectfeed efficiencyen
dc.subjectintestinal developmenten
dc.subjectanaerobesen
dc.titlePioneer colonizers: Bacteria that alter the chicken intestinal morphology and development of the microbiotaen
dc.title.serialFrontiers in Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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