Murgantia histrionica (Hahn): new trapping tactics and insights on overwintering survival

Harlequin bugs are orange and black aggregation pheromone emitting stink bug pests, specifically of cole crops such as kale, broccoli and collards. This nearly loyal crop preference makes an interesting challenge for trapping them and helping farmers predict pest severity. Harlequin bugs can be found in much of North America, and are a serious problem in the southeastern United States. Presumably their persistence into northern regions is limited by extreme winters. In 2014 and 2015 the arctic polar vortex extended into mid-latitudes bringing a blanket of sustained sub-freezing temperatures to much of the United States. We used these events to determine effects of extreme winter weather on harlequin bug survival. In both years we observed nearly identical low temperatures of -15oC and this linked to high (80-96%) harlequin bug mortality. In the lab we measured exact lethal freezing temperatures in harlequin bugs (i.e. supercooling points) to see if a physiological metric could be used to predict overwinter survival. Harlequin bug adults froze and died at -10.4oC, and similarly, their larger juvenile stages freeze at -11.0oC. Freshly hatched harlequin bugs and unhatched eggs froze at considerably lower temperatures with eggs forming ice crystals at -23.2oC and recent hatches at -21.6oC. Now with an understanding of how harlequin bugs likely survive winter extreme, we can then work on developing a trap to tally their populations in the spring and predict summer and fall pest severity. In the lab and field, harlequin bug adults and large nymphs were more likely found on green and black colors, and statistically less frequently on yellow, white, purple or red colors with the exception of adult females, which were most attracted to red and green in the lab, but green and black in the field. To increase harlequin bug attraction to and termination at traps square corrugated plastic panels were wrapped with an insecticide netting and baited with harlequin bug aggregation pheromone, murgantiol. Bugs were effectively drawn to the panels, with green panels having significantly more dead harlequin bugs and fewer dead beneficial lady beetles (Coleoptera: Coccinellidae) at their base than yellow panels. Thus, green was chosen as the ideal trap color to use for another field experiment that evaluated three trap types -- a corrugated plastic square panel, pyramidal trap, and ramp trap -- each with three lure treatments, murgantiol alone or murgantiol plus a low or high rate of mustard oil. More bugs were killed with the pyramidal trap than with the panel trap or the ramp trap, and more bugs were killed at traps containing murgantiol combined with benzyl isothiocyanate than at those with murgantiol alone. This research demonstrated that with the proper visual elements and odors, harlequin bugs can be drawn to traps and effectively killed after contact with insecticide-incorporated netting.