Method and device for annihilation of methicillin-resistant Staphylococcus aureus

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dc.contributor.authorCheng, J. X.en
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorDong, P. T.en
dc.contributor.authorHui, J.en
dc.contributor.inventorCheng, J. X.en
dc.contributor.inventorSeleem, Mohamed N.en
dc.contributor.inventorDong, P. T.en
dc.contributor.inventorHui, J.en
dc.date.accessioned2021-12-17T16:04:08Zen
dc.date.available2021-12-17T16:04:08Zen
dc.date.issued2021-05-25en
dc.date.updated2021-12-17T16:04:07Zen
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) possesses array of strategies to evade antibiotics through mutational inactivation, hiding inside host immune cells or concealing inside the biofilm in a sessile form. We report a drug-free approach to eradicate MRSA through blue-light bleaching of staphyloxanthin ( STX ), an anti-oxidative carotenoid residing inside the cell membrane of S. aureus. The photobleaching process, uncovered through a transient absorption imaging study and quantitated by mass spectrometry, decomposes STX and sensitizes MRSA to reactive oxygen species attack. Consequently, photobleaching using low-level blue light exhibits high-level synergy when combined with low-concentration of hydrogen peroxide. Anti microbial effectiveness of this synergistic therapy is validated in MRSA culture, MRSA-infected macrophage cells, biofilm, and a mouse wound infection model. Collectively, these findings highlight broad applications of STX photo bleaching for MRSA-infected diseases.en
dc.format.mimetypeapplication/pdfen
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.patentnumber11,013,933en
dc.identifier.urihttp://hdl.handle.net/10919/107094en
dc.language.isoenen
dc.publisherUS Patenten
dc.relation.urihttps://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PALL&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&r=1&f=G&l=50&s1=11,013,933.PN.&OS=PN/11,013,933&RS=PN/11,013,933en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleMethod and device for annihilation of methicillin-resistant Staphylococcus aureusen
dc.typePatenten
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.start-date2021-05en

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