Trypanosoma cruzi strain and starvation-driven mitochondrial RNA editing and transcriptome variability

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dc.contributor.authorGerasimov, Evgeny S.en
dc.contributor.authorRamirez-Barrios, Rogeren
dc.contributor.authorYurchenko, Vyacheslaven
dc.contributor.authorZimmer, Sara L.en
dc.date.accessioned2023-07-21T19:40:28Zen
dc.date.available2023-07-21T19:40:28Zen
dc.date.issued2022-07-01en
dc.date.updated2023-07-21T11:33:46Zen
dc.description.abstract<i>Trypanosoma cruzi</i> is a unicellular protistan parasitic species that is comprised of strains and isolates exhibiting high levels of genetic and metabolic variability. In the insect vector, it is known to be highly responsive to starvation, a signal for progression to a life stage in which it can infect mammalian cells. Most mRNAs encoded in its mitochondrion require the targeted insertion and deletion of uridines to become translatable transcripts. This study defined differences in uridineinsertion/ deletion RNA editing among three strains and established the mechanism whereby abundances of edited (and, thus, translatable) mitochondrial gene products increase during starvation. Our approach utilized our custom T-Aligner toolkit to describe transcriptome-wide editing events and reconstruct editing products from high-throughput sequencing data. We found that the relative abundance of mitochondrial transcripts and the proportion of mRNAs that are edited varies greatly between analyzed strains, a characteristic that could potentially impact metabolic capacity. Starvation typically led to an increase in overall editing activity rather than affecting a specific step in the process. We also determined that transcripts CR3, CR4, and ND3 produce multiple open reading frames that, if translated, would generate different proteins. Finally, we quantitated the inherent flexibility of editing in T. cruzi and found it to be higher relative to that in a related trypanosomatid lineage. Over time, new editing domains or patterns could prove advantageous to the organism and become more widespread within individual transcriptomes or among strains.en
dc.description.versionPublished versionen
dc.format.extentPages 993-1012en
dc.format.extent20 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1261/rna.079088.121en
dc.identifier.eissn1469-9001en
dc.identifier.issn1355-8382en
dc.identifier.issue7en
dc.identifier.orcidRamirez Barrios, Roger [0000-0002-4724-6724]en
dc.identifier.otherrna.079088.121 (PII)en
dc.identifier.pmid35470233en
dc.identifier.urihttp://hdl.handle.net/10919/115816en
dc.identifier.volume28en
dc.language.isoenen
dc.publisherCold Spring Harbor Laboratory Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000812825700006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectChagas diseaseen
dc.subjectRNA editingen
dc.subjectmetabolismen
dc.subjectepimastigoteen
dc.subjectelectron transport chainen
dc.subjectCYTOCHROME-OXIDASEen
dc.subjectMINICIRCLE GENOMEen
dc.subjectGENEen
dc.subjectDIFFERENTIATIONen
dc.subjectMECHANISMen
dc.subjectHuman Genomeen
dc.subjectVector-Borne Diseasesen
dc.subjectInfectious Diseasesen
dc.subjectGeneticsen
dc.subjectBiotechnologyen
dc.subject.meshAnimalsen
dc.subject.meshMammalsen
dc.subject.meshTrypanosoma brucei bruceien
dc.subject.meshTrypanosoma cruzien
dc.subject.meshProtozoan Proteinsen
dc.subject.meshRNAen
dc.subject.meshRNA, Messengeren
dc.subject.meshRNA, Protozoanen
dc.subject.meshRNA Editingen
dc.subject.meshTranscriptomeen
dc.subject.meshRNA, Mitochondrialen
dc.title<i>Trypanosoma cruzi</i> strain and starvation-driven mitochondrial RNA editing and transcriptome variabilityen
dc.title.serialRNAen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2022-04-07en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen

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