Embedded Passivated-Electrode Insulator-Based Dielectrophoretic Chromatography

Abstract

The detection and identification of particles within fluid samples is key in the prevention of the spreading of disease. This has created a growing need for devices able to successfully separate and identify multiple particles for this purpose while operating at a high enough throughput to be applicable in the field. A well investigated method of manipulating particles in this way is Dielectrophoresis (DEP), which is the use of varied electric fields gradients to generate a force on small particles. The strength of DEP depends of the properties of the particle medium, the signal generating the electric field, and the properties of the particles themselves. This method and its interaction with all small particles, including biological particles such as blood and cancer cells, has allowed devices utilizing this idea to be investigated for various biological purposes. This thesis investigates methods to increase the throughput of these types of devices in order to increase their ability to process large amounts of samples in reasonable amounts of time. This is done in primarily two methods. One approach uses the application of chromatographic methods to DEP devices to separate particles by altering their individual transit time through a device, allowing identification during constant flow. Another method is through mass parallel channels which each individually operate as a standard DEP particle trapping device. This allows for the summation of the maximum flow through the device due to its design layout.