Fasting differentially alters the hypothalamic proteome of chickens from lines with the propensity to be anorexic or obese

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dc.contributor.authorLiu, Lingbinen
dc.contributor.authorYi, Jiaqingen
dc.contributor.authorRay, W. Keithen
dc.contributor.authorVu, Lucas T.en
dc.contributor.authorHelm, Richard F.en
dc.contributor.authorSiegel, Paul B.en
dc.contributor.authorCline, Mark A.en
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2019-07-24T17:19:07Zen
dc.date.available2019-07-24T17:19:07Zen
dc.date.issued2019en
dc.description.abstractBackground: The hypothalamus is the ultimate modulator of appetite and energy balance and therefore sensitive to changes in nutritional state. Chicks from lines selected for low (LWS) and high (HWS) body weight are hypophagic and compulsive eaters, respectively, and differ in their propensity to become obese and in their hypothalamic mRNA response to fasting. Methods: As fasting-induced changes in hypothalamic proteins are unknown, we investigated the hypothalamic proteomes of 5-day old LWS and HWS chicks in the fed and fasted states using a label-free liquid chromatographytandem mass spectrometry (LC-MS/MS) approach. Results: A total of 744 proteins were identified in the chicken hypothalamus, and 268 differentially abundant proteins were identified among four pairwise comparisons. Ninety-five proteins were associated with the response to fasting in HWS chicks, and 23 proteins were associated with the response to fasting in LWS chicks. Fasting-responsive proteins in HWS chicks were significantly enriched in ATP metabolic processes, glyoxylate/dicarboxylate metabolism, and ribosome function. There was no enrichment for any pathways in LWS chicks in response to fasting. In the fasted and fed states, 159 and 119 proteins differed between HWS and LWS, respectively. Oxidative phosphorylation, citric acid cycle, and carbon metabolism were the main pathways associated with differences between the two lines of chicks. Enzymes associated with metabolic pathways differed between HWS and LWS in both nutritional states, including fumarase, aspartate aminotransferase, mitochondrial GOT2, 3-hydroxyisobutyrate dehydrogenase, chondrogenesis associated lipocalin, sialic acid synthase, arylamine N-acetyltransferase, pineal gland isozyme NAT-3, and succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial. Conclusions: These results provide insights into the hypothalamic metabolic pathways that are affected by nutritional status and the regulation of appetite and eating behavior.en
dc.description.sponsorshipThis research was supported by grant funding from Virginia’s Commonwealth Health Research Board and in part, by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture. Mass spectrometry resources used in this work aremaintained in part through funding by the Fralin Life Science Institute, the Agricultural Experiment Station Hatch Program, and the McIntire-Stennis Program at Virginia Tech.en
dc.format.extent13 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLiu et al. Nutrition and Diabetes (2019) 9:13 https://doi.org/10.1038/s41387-019-0081-1en
dc.identifier.doihttps://doi.org/10.1038/s41387-019-0081-1en
dc.identifier.urihttp://hdl.handle.net/10919/91964en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleFasting differentially alters the hypothalamic proteome of chickens from lines with the propensity to be anorexic or obeseen
dc.title.serialNutrition and Diabetesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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