Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds

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dc.contributor.authorRafael, Míriam Silvaen
dc.contributor.authorBridi, Leticia Cegattien
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorMarinotti, Osvaldoen
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorTimoshevskiy, Vladimiren
dc.contributor.authorGuimarães-Marques, Giselle Mouraen
dc.contributor.authorSantos, Valéria Silvaen
dc.contributor.authorda Silva, Carlos Gustavo Nunesen
dc.contributor.authorAstolfi-Filho, Spartacoen
dc.contributor.authorTadei, Wanderli Pedroen
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2021-02-26T19:56:34Zen
dc.date.available2021-02-26T19:56:34Zen
dc.date.issued2021-02-15en
dc.date.updated2021-02-26T14:42:18Zen
dc.description.abstractThe genome assembly of <i>Anopheles darlingi</i> consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among <i>Anopheles</i> species. <i>Anopheles darlingi</i> genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the <i>An. darlingi</i> scaffolds with homologous sequences of the <i>Anopheles albimanus</i> and <i>Anopheles gambiae</i> genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRafael, M.S.; Bridi, L.C.; Sharakhov, I.V.; Marinotti, O.; Sharakhova, M.V.; Timoshevskiy, V.; Guimarães-Marques, G.M.; Santos, V.S.; da Silva, C.G.N.; Astolfi-Filho, S.; Tadei, W.P. Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds. Insects 2021, 12, 164.en
dc.identifier.doihttps://doi.org/10.3390/insects12020164en
dc.identifier.urihttp://hdl.handle.net/10919/102455en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectin situ hybridizationen
dc.subjectgenomics and cytogeneticsen
dc.subjectsyntenyen
dc.subjectpolytene chromosomeen
dc.titlePhysical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffoldsen
dc.title.serialInsectsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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