Effect of concentration of glutaraldehyde and glyoxal on binding lysozyme to zein based films to control foodborne pathogens in tomatoes

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dc.contributor.authorRichter, Kevinen
dc.contributor.committeechairMallikarjunan, Parameswaran Kumaren
dc.contributor.committeememberKrometis, Leigh-Anne H.en
dc.contributor.committeememberWilliams, Robert C.en
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2014-03-14T21:32:47Zen
dc.date.adate2012-05-03en
dc.date.available2014-03-14T21:32:47Zen
dc.date.issued2012-02-16en
dc.date.rdate2012-05-03en
dc.date.sdate2012-04-06en
dc.description.abstractThe demand for biodegradable packaging materials as an alternative to synthetic ones to reduce environmental cost has seen an increase in recent years. In addition, functionalizing the packaging film to provide specific advantages like antimicrobial properties has yet to be explored thoroughly. This study considers adding antimicrobial agents to improve the quality and safety of actively packaged fresh produce using an antimicrobial enzyme (lysozyme) immobilized on a biopolymer based packaging film (corn-zein). The developed packaging material is aimed as an active biodegradable packaging to reduce bacterial contamination on the surface of fresh organic produce, specifically tomatoes. The study uses glutaraldehyde and glyoxal as binding agents to immobilize the enzyme on the packaging film. The effect of concentration of glutaraldehyde and glyoxal on the controlled release of the enzyme was studied. Concentrations of 0.1 and 0.2 g/g lysozyme: cross linking agent had controlled release properties. However, concentrations of 0 or 0.05 are about 20-30% more effective at inactivating bacteria. Antimicrobial activity in the constructed zein films are also tested against selected pathogens (Salmonella Newport and Listeria monocytogenes). Developed zein based film is tested against inoculated tomatoes to determine the efficacy of the films in reducing the pathogen population. The inoculated tomatoes are stored at room temperature over a storage period of one week. The film was able to reduce Listeria monocytogenes population by three logs but was unable to reduce the population of Salmonella Newport.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-04062012-133842en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04062012-133842/en
dc.identifier.urihttp://hdl.handle.net/10919/41893en
dc.publisherVirginia Techen
dc.relation.haspartRichter_K_T_2012.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectGlyoxalen
dc.subjectGlutaraldehydeen
dc.subjectLysozymeen
dc.subjectFilmsen
dc.subjectZeinen
dc.subjectActive Packagingen
dc.subjectTomatoesen
dc.subjectCross linkingen
dc.subjectFood Safetyen
dc.subjectListeriaen
dc.subjectSalmonellaen
dc.titleEffect of concentration of glutaraldehyde and glyoxal on binding lysozyme to zein based films to control foodborne pathogens in tomatoesen
dc.typeThesisen
thesis.degree.disciplineBiological Systems Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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