The Role of Angiotensin II in Skeletal Muscle Metabolism

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dc.contributor.authorWahlberg, Kristinen
dc.contributor.committeechairHulver, Matthew W.en
dc.contributor.committeememberLi, Liwuen
dc.contributor.committeememberFrisard, Madlyn I.en
dc.contributor.committeememberDavy, Kevin P.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2014-03-14T21:35:39Zen
dc.date.adate2011-06-13en
dc.date.available2014-03-14T21:35:39Zen
dc.date.issued2011-04-27en
dc.date.rdate2011-06-13en
dc.date.sdate2011-05-05en
dc.description.abstractHypertension and diabetes have long been closely linked. As such, the major player in the renin, angiotensin system, angiotensin II, has recently been investigated for its effects on metabolism and diabetes. Since skeletal muscle is one of the most metabolically active tissues, this study investigates the effects of angiotensin II specifically on skeletal muscle. In this study, L6 skeletal muscle cells were treated with angiotensin II for either 3 or 24 hours and a number of effects were investigated. Fatty acid oxidation and lipid synthesis was measured using [1-14C]-palmitate, glucose oxidation and glycogen synthesis were measured using 14C-glucose. In addition,mitochondrial oxidative capacity was measured using an XF 24 Flux Analyzer (Seahorse Bioscience) and reactive oxygen species measured using confocal microscopy. The clinical study involving the drug Benicar ® investigated the metabolic effects of blocking angiotensin II on skeletal muscle fatty acid oxidation, glucose oxidation, and oxidative and glycolytic enzyme activity. In L6 cells, angiotensin II significantly reduced fatty acid oxidation after 24 hours (p<0.01) and 3 hours (p<0.001) if angiotensin II was present during oxidation experiments. It also significantly reduced mitochondrial oxidative capacity (p<0.05) after 24 hours and significantly increased reactive oxygen species production (p<0.05) over 3 hours. The clinical study showed no significant effects of Benicar® on fatty acid or glucose oxidation or any enzyme activities.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05052011-103310en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05052011-103310/en
dc.identifier.urihttp://hdl.handle.net/10919/42504en
dc.publisherVirginia Techen
dc.relation.haspartWahlberg_KA_T_2011.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectROSen
dc.subjectOxidationen
dc.subjectskeletal muscleen
dc.subjectangiotensin IIen
dc.titleThe Role of Angiotensin II in Skeletal Muscle Metabolismen
dc.typeThesisen
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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