Response of Leaf Protein to Ozone in Two White Clover Clones

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dc.contributor.authorYoulin, Tang M.S.en
dc.contributor.committeechairChevone, Boris I.en
dc.contributor.committeememberMoore, Laurence D.en
dc.contributor.committeememberHess, John L.en
dc.contributor.committeememberChevone, Boris I.en
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen
dc.date.accessioned2014-03-14T21:52:56Zen
dc.date.adate1998-05-27en
dc.date.available2014-03-14T21:52:56Zen
dc.date.issued1998-05-07en
dc.date.rdate1999-05-27en
dc.date.sdate1998-05-07en
dc.description.abstractA white clover ( Trifolium repens ) system, based on one ozone-sensitive (NC-S) and one ozone-resistant (NC-R) clone, has been developed as an indicator to estimate the effects of tropospheric ozone on plant biomass production. A reduction in the vegetative biomass ratio (NC-S/NC-R) of the clones was correlated with increasing concentrations of ozone during a 28-day exposure period. However, the mechanism of ozone sensitivity or tolerance at the biochemical or molecular level is not known. Superoxide dismutase isozyme activities in the two clones did not respond differently to ozone treatment. However, catalase activity increased somewhat more in the leaf tissue of NC-R, compared to NC-S, after ozone treatment. Two-dimensional polyacrylamide gel electrophoresis (PAGE) showed the presence of two proteins (Ozone-Response-Proteins, ORPs) that were more responsive to ozone in the tolerant genotype than in the sensitive one. After ozone treatment for three days, the ORPs were four-fold higher in leaf tissue of NC-R compared to NC-S. Also, the amount of the ORPs was twenty-fold higher in leaf tissue of ozone-treated NC-R than in that of control NC-R. These proteins have apparent molecular weights of 21.5 kD and 23 kD and isoelectric points of 4.1-4.4 on SDS-PAGE gels. The filtrate of a 100 kD concentrator showed that the native molecular weights of the ORPs were less than 100 kD. The results obtained from a study of field samples demonstrated that protein content in leaf tissue of both NC-R and NC-S was positively correlated with ORP content.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-42398-124213en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-42398-124213/en
dc.identifier.urihttp://hdl.handle.net/10919/46494en
dc.publisherVirginia Techen
dc.relation.haspartDissertation-1.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectWhite Cloveren
dc.subjectOzone-Responsive Proteinen
dc.titleResponse of Leaf Protein to Ozone in Two White Clover Clonesen
dc.typeThesisen
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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