Effects of using an enzyme-linked immunosorbent assay to monitor the control of Staphylococcus aureus mastitis in dairy herds

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dc.contributor.authorGrove, Tina Moleren
dc.contributor.committeechairJones, Gerald M.en
dc.contributor.committeememberBarnes, Michael A.en
dc.contributor.committeememberBishop, J. Russellen
dc.contributor.committeememberVinson, William E.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T21:33:51Zen
dc.date.adate2009-04-14en
dc.date.available2014-03-14T21:33:51Zen
dc.date.issued1990-07-05en
dc.date.rdate2009-04-14en
dc.date.sdate2009-04-14en
dc.description.abstractBovine mastitis is the most important economic disease to the dairy industry with losses estimated at 2 billion dollars per year in the United States. Staphylococcus aureus (.§.. aureus) is the primary cause of contagious mastitis. Conventional culture methods (National Mastitis Council) were used as a basis for comparing the ability of the enzyme linked immunosorbent assay. ProStaph Iâ ¢, to identify s. aureus. The test had an accuracy of 96%, with a sensitivity of 90% and a specificity of 97%. Results indicated that rinsing teat-cup liners with a 25 ppm iodophor or 100 ppm chlorine solution reduced the presence of S. aureus on the liners by 97%. ProStaph I was used to rapidly screen DHIA preserved milk samples in 10 Virginia cooperator herds. Herds were classified as high (>10% infected) or low prevalence (<10% infected). There were six high prevalence herds after the first test. Average prevalence of cows scoring Ab +2 and +3 was 11.9% ± 7.9. Over the seven month study, prevalence of positive cows declined significantly (P<.OI), but somatic cell count remained relatively unchanged (P>.lO). Four herds continued to have >10% of the animals infected. Incidence of new infection averaged 3.6% ± 2.8 from the first to the last test. Chronic cows averaged 6.9% ± 4.8 over the seven month study. Analysis of variance showed significant (P<.Ol) effects of herd on ProStaph I score J milk yield, and see. Elevated ProStaph I scores were highly correlated (P <.01) with increases in lactation number. ProStaph I changed quadratically (P<.Ol) with increasing SCC. Somatic cell count increased (P<.OI) as ProStaph I score increased.en
dc.description.degreeMaster of Scienceen
dc.format.extentx, 77 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-04142009-040817en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04142009-040817/en
dc.identifier.urihttp://hdl.handle.net/10919/42101en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1990.G775.pdfen
dc.relation.isformatofOCLC# 23116663en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1990.G775en
dc.subject.lcshEnzyme-linked immunosorbent assayen
dc.subject.lcshMastitis -- Diagnosisen
dc.subject.lcshStaphylococcus aureus -- Diagnosisen
dc.titleEffects of using an enzyme-linked immunosorbent assay to monitor the control of Staphylococcus aureus mastitis in dairy herdsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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